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RNAintro shRNA starter kits

shmarter RNAi...Guaranteed
  • All the tools for in vitro  or in vivo gene silencing in one box
  • Your choice of  shRNAmir constructs from the human or mouse genomes
  • Produce meaningful RNAi data with validated and optimized reagents
  • Guaranteed knockdown

The RNAintro shRNA starter kits combine the versatility and specificity of Expression Arrest™ lentiviral and retroviral shRNAmir, the simplicity of validated controls, and optimized transfection reagents- all in a guaranteed to work package. 

RNAintro kits for pSM2 retroviral shRNAmir include:
 

  • Three pSM2 shRNAmir constructs- Your choice from the human and mouse genomes
  • Arrest-In transfection reagent for shRNA delivery
  • Validated positive controls
  • Negative control- Non silencing shRNA construct
  • Transfection efficiency control- β gal reporter

RNAintro kits for GIPZ lentiviral shRNAmir include:  

  • Three GIPZ shRNAmir constructs- Your choice from the human genome
  • Arrest-In transfection reagent for shRNA delivery
  • Validated EG5 and GAPDH positive controls
  • Negative control- Non silencing shRNA construct 

These components have been validated and packaged along with detailed and simplified protocols to ensure the success of your gene silencing experiments.  Produce meaningful RNAi data with validated reagents and do so confidently with guaranteed to work shRNAmir constructs.
Open Biosystems...start working shmarter
These products are covered by a Limited Use License

Shipping Information:
pSM2 retroviral shRNAmir constructs are shipped as bacterial cultures of E.coli in LB broth with 8% glycerol and chloramphenicol (50 µg/ml). pGIPZ lentiviral shRNAmir containing vectors are shipped as bacterial cultures of E.coli  in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml) and zeocin (25ug/ml). Open Biosystems checks all cultures for growth prior to shipment. Individual constructs are shipped on wet ice. All Expression Arrest™ shRNA constructs should be stored at -80°C. Arrest-In™ transfection reagent should be stored at 4°C. Do not freeze. 
Simple steps to guaranteed gene silencing



 

Examples of successful gene silencing using the RNAintro pSM2 and GIPZ kits with shRNAmir directed against various targets. (A) pSM2 shRNAmir directed against mouse VEGF. Over 90% knockdown of VEGF protein was detected compared to non-silencing control. (B) p53, PTEN, RB, STAT6 and positive controls GAPDH and EG5 show significant knockdown compared to the non-silencing control.

Purchasing Information
Catalog No.
Description
Literature
RHS3600
RNAintro pSM2 retroviral shRNAmir starter kit-Luciferase
 
RHS3601
RNAintro pSM2 retroviral shRNAmir starter kit-eGFP
 
RHS4287
RNAintro GIPZ lentiviral shRNAmir starter kit
 

 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07