pLKO.1 Lentiviral shRNA libraries from the RNAi Consortium (TRC)

The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard, and includes six MIT and Harvard associated research institutions and five international life sciences organizations. Over three years TRC will create lentiviral shRNA libraries targeting 15,000 human (TRC-Hs 1.0) and 15,000 mouse (TRC-Mm1.0) annotated genes with a goal of validating each shRNA in multiple cell lines. Open Biosystems has partnered with the TRC to make these shRNA libraries available to researchers worldwide. Currently the TRC lentiviral shRNA libraries consist of approximately 63,500 human shRNA constructs targeting ~13K human genes and over 56,000 mouse shRNA constructs targeting ~12K mouse genes. Additional releases will occur every quarter.

    Features of the TRC shRNA libraries include:

• Rules-based shRNA design for efficient gene knockdown
• Already cloned into lentiviral vectors
• Amenable to in vitro and in vivo applications such as the creation of stable knockdowns
• Lentiviral vector enables transduction of primary and non-dividing cell lines
• Broad coverage: 4-5 constructs per gene

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Ordering shRNA

Enter your gene symbol(s) or GenBank accession number(s) into the clone query ( )
Results flanked by a green icon are exact matches to your search criteria
Click on the details link for additional information including hairpin sequences
Order directly online. Constructs are shipped in 48 hours
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you.