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Mouse shRNAmir retroviral libraryComplete Genome-wide coverage
The mouse retroviral shRNAmir genome-wide library covers
~28,000 mouse genes with multiple constructs/gene. Currently the library
consists of 61,000 shRNAmir constructs and broadly covers the
mouse genome. The library will continue to grow, adding deeper coverage
to genes currently represented and adding the few genes not already
represented.
The mouse library can be purchased as individual releases 2.3-2.16 or the entire library (subscription). Each individual release contains~ 4800 constructs randomly arranged into 96 well microtiter plates. The mouse retroviral shRNAmir library has been functionally annotated into biologically relevant gene families and pathways. The pie chart shows coverage by functional group (Silva et al 2005).
The shRNAmir genome wide libraries and rearrays of gene
families are provided in 96-well microtiter plates containing frozen
stock cultures of E. coli
(DH10bpir116) in LBR broth with 8% glycerol and chloramphenicol
(50ug/ml). Open Biosystems checks all cultures for growth prior to
shipment. Individual shRNAmir constructs are shipped as
glycerol stock cultures on wet ice. Store at -80C
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
