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MicroRNA-adapted Retroviral Vectorsfor flexible shRNAmir cloning and expression
A series of microRNA-adapted retroviral vectors have been recently
developed in the laboratory of Scott Lowe at CSHL to enable the
efficient expression of shRNAmir constructs from RNA Polymerase II
(Pol II) promoters (Dickens et al 2005). These vectors produce
highly efficient knockdown even when present at single copy in the
genome. Since Pol II transcribes endogenous primary microRNA
transcripts, the improved performance of these vectors seems to
derive from natural mechanisms of RNA-dependent gene
inhibition. Features of the vectors include:
This product is covered by a Limited Use License
Each vial of Expression Arrest Pol II retroviral vectors is shipped at a
concentration of 0.5µg/µl in a total volume of 20µl, thus providing a
total amount of 10µg vector DNA. The vector DNA is shipped in a
microfuge tube at room temperature and should be stored at -20°C or
-80°C.
Dickens NG et al (2005) "Probing tumor phenotypes using stable
and regulated synthetic microRNA precursors" Nature Genetics Vol 37, No
11 1289-1295.
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