Human shRNAmir retroviral libraryComplete Genome-wide coverage
The human retroviral shRNAmir genome-wide library covers 28,500 human
genes with multiple constructs/gene. Currently the library consists of
81,500 shRNAmir constructs and will continue to grow, adding deeper
coverage to genes currently represented and adding the few genes not
already represented.
The human library can also be purchased as individual releases 1.3-1.19. Each individual release contains~ 4800 constructs randomly arranged into 96 well microtiter plates. The human shRNAmir library has been rearrayed into functionally annotated Gene Families and pathways. The pie chart shows coverage by functional group (Silva et al 2005). ![]()
The shRNAmir genome wide libraries and rearrays of gene families
are provided in 96-well microtiter plates containing frozen stock
cultures of E. coli
(DH10bpir116) in LBR broth with 8% glycerol and chloramphenicol
(50ug/ml). Open Biosystems checks all cultures for growth prior to
shipment. Individual shRNAmir constructs are shipped as glycerol
stock cultures on wet ice. Store at -80ºC.
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