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	Germany	0241 6085 13140	0241 6085 33033
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Human ORF Collection

for easily moving thousands of open reading frames into your choice of protein expression systems

The Center for Cancer Systems Biology of the Dana-Farber Institute has created a path-breaking collection of ORF-only clones (full-length cDNAs with UTRs removed) beginning with over 8000 genes¹ and with future updates planned. These clones are derived from fully sequenced Mammalian Gene Collection (MGC) full-length cDNAs and cloned into a recombinational entry vector.

Time savings : ORF clones provide a shortcut to protein expression, allowing you to skip PCR, cloning into an expression vector, and verifying the ends of the ORF DNA sequence.
High fidelity : Each CDS was amplified for only 25 cycles with gene-specific primers and KOD HiFi Polymerase², greatly minimizing the risk of PCR-induced mutations.
Ease of transfer: The Gateway^® entry vectors ensure easy transfer into prokaryotic, mammalian, viral, or insect expression systems. For maximum flexibility, the ORF stop codon has been removed.

²KOD HiFi Polymerase is supplied by Novagen.
Gateway is a registered trademark of Invitrogen.

References:

¹Rual JR et al (2004). Human ORFeome Version 1.1: a platform for reverse proteomics. Genome Research 14, 2128–2135

²Rual, J-F et al (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature 437, 1173–1178

ORF Clone Application

Each human ORF may be transferred to a Gateway compatible destination vector by homologous recombination. The resulting destination construct is ready for ORF protein expression in a range of functional genomics applications, including yeast two-hybrid screens, protein expression and purification, and intracellular imaging.

Purchasing Information
Catalog No.	Description	Literature
OHS1770	Human ORF Clone Search the clone on the website of te supplier : Here
OHS1771	Human ORF Collection (Release 1)
OHS4105	BioTrack Set - Kinase (ORF)

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com

International	+32 (0) 16 58 90 45	+32 (0) 16 50 90 45
France	01 43 25 01 50	01 43 25 01 60
Italy	02 36 00 65 93	02 36 00 65 94
Germany	0241 6085 13140	0241 6085 33033

Human ORF Collection

Send mail to webmaster with questions or comments about this web site. Copyright © 2005 Gentaur BVBA Last modified: feb-07

Send mail to webmaster with questions or comments about this web site.
Copyright © 2005 Gentaur BVBA
Last modified: feb-07