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Fluorescent Arrest-InDual function reagent for shRNA delivery
Fluorescent (Fl) Arrest-In™ is sulforhodamine B-conjugated
Arrest-In™. It was
developed to enable visualization in real-time, of Arrest-In-DNA
complexes in cells. Fl Arrest-In™ is a dual function
reagent, permitting visualization of transfected complexes as well
as efficient delivery of shRNA into the nucleus of transfected cells.
Features include:
Double label studies with Green Fluorescent Protein (GFP)
The rhodamine fluorescent tag (excitation
is at 560nm and emission at 580nm) allows double label experiments with
a variety of commonly available tags (GFP, FITC, biotin etc.). Fl
Arrest-In has been tested on a variety of cell types and has shown
transfection efficiencies comparable to that seen using unconjugated
Arrest-In™. Studies to assay for cytotoxic effects of both Arrest-In and
Fl Arrest-In show that both reagents had minimal to no cytotoxic effects
when compared against untreated cells. See
product insert
for data.
Fl Arrest-In™ is supplied in sterile filtered water at a concentration
of 1 mg/ml. 0.5ml, 1ml, 5x1ml and 10x1ml sizes are available. Store at
4°C for upto 12 months. Do not freeze.
Pollard et al (1998)
Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to
the Nucleus in Mammalian Cells. Journal of Biological Chemistry, Vol
273: 13; 7507-7511.
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