|
|
FishFugu IMAGE cDNAs Fugu IMAGE cDNAs
Stickleback cDNA Collectionsan Open Labs Product for identifying tissue- and population-specific changes in gene expression
The three-spine stickleback (Gasterosteus
aculeatus), a teleost fish, is an evolutionary model for
morphological change that is growing in popularity. (It was mentioned under
Science's "Breakthrough of the Year" for 2005.) We offer 6 collections of
cDNAs originating from specific tissues in different geographical
populations.
Shipping Information:
For technical give and take with other members of the Stickleback research community, you are invited to post to the new Stickleback forum. Photo courtesy of the David Kingsley Lab (http://kingsley.stanford.edu/).
Individual clones:
References:
Clones are provided as a live culture in a 2-ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C. Clone Collections: Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
Culotta E and E Pennisi
(2005). Evolution in action. Science
310, 1878–1879
Zebrafish Exelixis cDNAs
Zebrafish Gene Collection (ZGC)The Zebrafish Gene Collection (ZGC) is a top
priority NIH initiative that supports the production of cDNA libraries, clones
and sequences to provide a complete set of full-length cDNA clones of expressed
genes for zebrafish. The ZGC team is acquiring samples from multiple zebrafish
tissues and developmental states for construction of high-quality cDNA
libraries. These libraries are then 5’ end sequenced and the resulting EST
clones are reviewed by a panel of scientists. The reviewers flag candidate
full-length clones and prioritize them for sequencing. The candidate clones are
then subjected to full insert sequencing and the presence of a full open reading
frame is confirmed. Each clone is supplied in a plasmid vector transformed into
E. coli.
Zebrafish IMAGE cDNAs
The IMAGE consortium was formed in 1993 to provide a public resource to
aid in gene discovery that would be free of patents/royalties and other
restrictions1. The IMAGE clone collection provides the
community a resource of arrayed cDNA clones from various research
efforts. The clones are from oligo dT-primed, directionally cloned
plasmid cDNA libraries from human, mouse, rat, zebrafish, Xenopus
and primate sources. The Cancer Genome Anatomy Project (CGAP) was formed
in 1996 to investigate genes associated with cancerous cells at the
molecular level. As a result, CGAP is responsible for thousands of
expressed sequence tags (ESTs), which are continuously added to the
IMAGE collection. All of these clones can be ordered online through our
clone query.
Individual clones:
Clones are provided as a live culture in a 2ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2-3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.
Clone Collections:
Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
1The IMAGE Consortium: An Integrated Molecular Analysis of
Genomes and Their Expression (1996) Genomics, 33:151-2
Zebrafish IMCB CollectionSingapore's Institute for Molecular and Cell
Biology (IMCB) has isolated over 15,000 unique genetic markers from the
zebrafish (Danio rerio). A total of 15,590 unique zebrafish EST clusters
from two cDNA libraries (primary and normalized) have been identified. This EST
set is complementary to the existing ESTs in the public database and will be
invaluable in assisting in the annotation of genes based on the upcoming
zebrafish genome sequence. Their use in microarray studies to profile gene
expression patterns, especially in embryogenesis, will provide a quick and
inexpensive way to discover new genes and understand their regulation in the
body.
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Purchasing Information | ||
|
Catalog No.
|
Description
|
Literature
|
|
PZF1245
|
Zebrafish Morpholino
|
 |
| Purchasing Information | |||
|
Catalog No.
|
Description
|
Literature
|
Search
|
|
EDR4009
|
cDNA: NEI zebrafish clone
|
|
 |
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45