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Drosophila RNAi Library-dsRNAReady-to-go for RNAi screening
The Expression Arrest™ Drosophila RNAi library-dsRNA constructs
targets over 15,000 Drosophila genes with dsRNA constructs ready-to-go
for RNAi experiments.
Features of this library include:
The Drosophila RNAi-dsRNA library was developed by performing in
vitro transcription reactions on the Drosophila RNAi-dsDNA template
library. The library was validated by using a subset of the
dsRNA constructs produced for knockdown studies. These RNAi studies were
performed by and in collaboration the O'Farrell and Vale labs at UCSF. A
test plate for the dsRNA library is available. This test plate contains
8 genes with well known RNAi phenotypes as well as a non-silencing
control.
Drosophila
RNAi-dsRNA libraries are available in 96-well PCR plates. Plates
containing frozen constructs are shipped on dry ice. For long term
storage it is recommended to store the dsRNA at -70C. The dsRNA product
can be stored at -20C for at least 3 months without any sign of dsRNA
degradation.
Methodological Papers:
Carolyn A. Worby, Nancy Simonson-Leff, and Jack E. Dixon (2001) RNA Interference of Gene Expression (RNAi) in Cultured Drosophila Cells. Sci. STKE, 95, pl1. Armknecht S, Boutros M, Kiger A, Nybakken K, Mathey-Prevot B, Perrimon N. (2005) High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymol, 392: 55-73 Christophe J. Echeverri and Norbert Perrimon.(2006) High-throughput RNAi screening in cultured cells: a user's guide. Nature Reviews Genetics 7, 373-384.
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