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Drosophila RNAi library-DNA templates

High throughput RNAi screening
The Expression Arrest™ Drosophila RNAi Library-dsDNA templates is a collection of over 15,000 dsDNA templates ready for in vitro transcription (IVT) to dsRNA constructs that target the entire Drosophila genome.  dsDNA templates were generated using gene specific primers to amplify 200-800 bases of exonic sequence for each gene. Features include:
  • dsDNA templates for targeting the entire Drosophila genome
  • Perform high-throughput RNAi screening in Drosophila cells
  • Dual T7 promoters for direct use in in vitro transcription

  • Sufficient material to generate over 600ugs of dsRNA 

    The Drosophila RNAi Library 1.0 and 2.0 collectively target the entire Drosophila genome with dsDNA templates ready for generation of dsRNA constructs for RNAi. The dsDNA templates can successfully generate microgram quantities of dsRNA in a single IVT reaction. Using five randomly picked constructs from our collection with two commercially available kits, IVT reactions were performed. The RNA obtained ranged between 21 and 67 µg, using as little as 1ul of the template. The variation in yield is both template and kit dependent. With the 10µl of dsDNA provided, over 600 µg of RNA can be generated. The variation in yield is both template and kit dependent. Most protocols that involve microinjections, cell culture or transfection of RNAi in Drosophila require 0.1µg to 30µg of dsRNA. Download the pdf for a copy of the complete experiment.

    The Drosophila RNAi library is also available as a ready-to-go dsRNA construct library

     

Shipping Information:
Drosophila RNAi Library 1.0 and 2.0 are available in a high-throughput format in 96-well PCR plates. Plates contain frozen constructs and are shipped on dry ice. Store at –20° C. Individual constructs (available from Drosophila RNAi library 1.0 only) are shipped on wet ice.
 
References:
Foley E, O’Farrell PH (2004) "Functional dissection of an innate immune response by a genome-wide RNAi screen". PLoS Biol 2(8): e203.
Echard A., et al (2004) "Terminal cytokinesis events uncovered after an RNAi screen" Current Biology (14) 1685-1693.
 

Open Biosystems would like to acknowledge the following people for the development of this resource: Pat O’Farrell, Ron Vale, Greame Davis, Edan Foley, Ben Eaton, Nico Stuurman, and Steve Rogers all of the University of California, San Francisco. Though the aforementioned researchers developed this resource, they are not responsible for the product distributed by Open Biosystems.

 

Purchasing Information
Catalog No.
Description
Literature
Search
RDM1189
Drosophila RNAi Library 1.0
 
 
RDM1190
Drosophila RNAi Construct
 
RDM4220
Drosophila RNAi Library 2.0
 
 
RDM4339
Drosophila RNAi Library- Release 1.0 and 2.0
 
 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07