DNA polymerasea novel enzyme with unusual propertiesRainbow DNA
Polymerase is a recombinant family B DNA polymerase cloned from the
hyperthermophilic marine archaeon Thermococcus sp. OGL-20P,
isolated from the Rainbow hydrothermal vent field in the Atlantic Ocean. It
is a robust, multi-purpose enzyme that possesses a proofreading 3’-5’
exonuclease with high fidelity and can be used in all applications involving
primer extension. More thermostable than Taq polymerase (3 x longer
half life at 95°C), Rainbow DNA Polymerase’s high extension rate and
processivity generate superior yields in short reaction times using a wide
range of template types and purity levels. DNA products are blunt ended.
Shipped on dry ice and stored at
–20°C.
References:
1 Rainbow DNA
Polymerase is manufactured by ExtremoZyme Inc., licensed by the University
of Alabama at Huntsville (Huntsville, AL), and distributed by Open
Biosystems, Inc.
2 One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP in 30 minutes at 74°C in the presence of Rainbow reaction buffer, 0.2 mM each dNTP, and 16.6 ng/µL primed M13mp18 substrate
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