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Bacteria

Brucella ORF Collection

C. jejuni ORF Collection

E. coli Promoters

Brucella ORF Collection

an Open Labs Product for gene functional analysis of an intracellular pathogen

Created by a consortium of laboratories1, the Brucella ORF Collection is comprised of 3091 ORFs that have been cloned into a Gateway® entry vector and are ready to be transferred into Gateway destination vectors for protein expression and functional analysis (Figure 1). For example, protein-protein interactions can be studied by transferring the collection to bait and prey plasmids for yeast two-hybrid screening or to a destination construct that expresses each ORF with an affinity, epitope, or fluorescent tag.

For technical give and take with other members of the Brucella research community, you are invited to post to the new Brucella forum. 
 
Shipping Information:
Brucella ORF clones are available as glycerol stocks. Individual clones are shipped in microtubes at room temperature, while the entire collection is shipped in 96-well plates on dry ice. Microtubes and plates should be stored at –80ºC.
References:
1Dricot A et al (2004). Generation of the Brucella melitensis ORFeome Version 1.1. Genome Research14, 2201–2206

Purchasing Information
Catalog No.
Description
Literature
OBM3502
Brucella ORF Collection
 
OBM3503
Brucella ORF Clone
 
 

C. jejuni ORF Collection
 

Jodi Parrish et al1 from the Center for Molecular Medicine and Genetics, and Department of Biochemistry and Molecular Biology, Wayne State University have cloned 1,619 ORFs from Campylobacter jejuni into E. coli using in vivo recombination. The ORFs that were chosen include 1654 ORFs that were predicted by the Sanger Institute2 , together with 31 additional ORFs predicted by TIGR (http://www.tigr.org/tigr-scripts/CMR2/CMRHomePage.spl). The availability of these ORFs circumvents a common rate-limiting step in proteomics, the generation of an appropriate predicated ORF clone set for use in proteome-scale micro-array analysis. Their use in micro-array studies to profile gene expression patterns will be invaluable to the research community and will provide a quick and inexpensive way to investigate genes and proteins and understand their function and regulation.

E. coli SURE clones containing the C. jejuni ORFs in the vector pTLJ03 are available from Open Biosystems. pTLJ03 is an expression vector that generates N-terminal GST-His-tagged fusion proteins from the C. jejuni ORF set. Plasmid maps, details, and sequences are either available below or at http://proteome.wayne.edu. Recombination sites were verified by sequencing, and most clones have been 5’ sequenced. The ORFs range from 45 – 4,020bp in length.

Note: pTLJ03 is not a high-copy number plasmid. The C. jejuni ORF clones are cultured in LB with 100ug/ml Ampicillin.

 
Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2-3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

 
Bulk Orders:
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

 
Entire Collection:
Orders for the entire C. jejuni collection are provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.
 
References:
1 Parrish J, Limjindaporn T, Hines JA, Liu J, Liu G, and Finley RL, Jr. High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli,J. Proteome Research, in press (2004).
 
2 Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, Basham D, Chillingworth T, Davies RM, Feltwell T, Holroyd S, Jagels K, Karlyshev AV, Moule S, Pallen MJ, Penn CW, Quail MA, Rajandream MA, Rutherford KM, van Vliet AH, Whitehead S, Barrell BG. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature. 2000 Feb 10;403(6770):665-8.
 
Purchasing Information
Catalog No.
Description
Literature
Search
OCJ1761
ORF: C. jejuni ORF Clone
 
OCJ1762
ORF: C. jejuni ORF Collection
 
 

 

E. coli Promoters
an Open Labs Product for investigating gene regulation

Researchers at the Weizmann Institute of Science have produced a collection of E. coli strains that enables monitoring of gene expression at high temporal resolution in living cells. Each of the reporter strains has a bright, fast-folding green fluorescent protein (GFP) fused to a full-length copy of an E. coli promoter in a low-copy plasmid.

This collection includes more than 1900 promoters (out of 2500 in the entire genome) for E. coli K12 strain MG1655 and enables measurement of gene expression at a resolution of minutes with high accuracy and reproducibility. Performing experiments in a multi-well fluorimeter using FACS or time-lapse fluorescence microscopy has the necessary sensitivity to measure gene expression in individual cells.

For technical give and take regarding E. coli promoters, you are invited to post in the forum. 

 
Shipping Information:
Individual clones:
Clones are provided as a live culture in a 2-ml tube. Each tube contains LB medium supplemented with 8% glycerol and kanamycin. Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.

 
Bulk Orders:
Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

 
Entire Collection:
Orders for the entire E. coli Promoter Collection are provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.
 

 
References:
Kalir S et al (2001). Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080–2083

Ronen M et al (2002). Assigning numbers to the arrows: parameterizing a gene regulation network by using accurate expression kinetics. Proc. Natl. Acad. Sci. USA 99, 10555–10560

Setty et al (2003). Proc. Natl. Acad. Sci. USA 100, 7702–7707

Zaslaver A et al (2004). Just-in-time transcription program in metabolic pathways. Nature Genetics 36, 486–491
Purchasing Information
Catalog No.
Description
Literature
Buy
PEC3876
E. coli Promoter Clone
   
PEC3877
E. coli Promoter Collection
   
 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07