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Arrest-In transfection reagentMaximum shRNA delivery into the nucleus
Gene silencing using RNAi is critically dependent on highly efficient
delivery of shRNA or siRNAs into cells. It is well known that polymers,
but not cationic lipids, protect DNA in the cytoplasm and promote entry
into the nucleus (1) of transfected cells.
Arrest-In™ transfection reagent is a proprietary lipo-polymeric formulation, developed and optimized for transfection of shRNA plasmid DNA into the nucleus of cultured eukaryotic cells. Arrest-In also provides an enhanced uptake efficiency of the shRNA plasmid DNA into cells. Once in the cells, Arrest-In promotes the entry of the shRNA containing plasmid into the nucleus where it is transcribed into a hairpin, enters the cytoplasm, is processed by the endogenous RNAi machinery into functional siRNAs. Features include:
Arrest-In™ is supplied in sterile filtered water at a concentration of 1
mg/ml. 0.5ml, 1ml, 5x1ml and 10x1ml sizes are available. Store at 4°C
for upto 12 months. Do not freeze.
1. Pollard et al (1998)
Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the
Nucleus in Mammalian Cells. Journal of Biological Chemistry, Vol 273: 13;
7507-7511.
^Trademark information
Lipofectamine 2000™-Invitrogen ExGen 500™- Fermentas Fugene™-Roche
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