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HIV-1 BaL PURIFIED VIRAL LYSATE
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PRODUCT DESCRIPTION: |
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HIV-1 BaL virus is propagated in PM-1 cells, a clonal derivative of
Hut78 which allows for the growth of macrophage and T-cell tropic
viruses. The virus is purified using density gradient
ultracentrifugation, disrupted in the presence of detergent and
characterized at the biochemical and immunologic levels to assure
product consistency and performance.
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PRODUCT
COMPOSITION: |
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Purified HIV-1 (density 1.16g/cm3) is disrupted in 0.5% Triton X-100
nonionic detergent/0.6M KCI. Detergent is removed by 2 cycles of
ether extraction and the virus is subjected to in vitro
testing to ensure lack of infectivity. Each lot of purified virus is
examined for purity using SDS-PAGE and for antigen composition using
Western immunoblotting (WIB). Diagnostic levels of env
proteins (gp160, gp120 and gp41), gag proteins (p17, p24,
p39, p55) and pol gene-derived proteins (p31, p51/p66) are
present in the virus. The total protein concentration is determined
by Pierce BCA assay. Verification of virus inactivation is
determined by Reverse Transcriptase Reduction Assay.
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RECOMMENDED USES: |
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Immunodetection of antibodies to HIV-1 using solid-phase enzyme
immunoassay (EIA), WIB, dot-blotting and related techniques. The
material should also be of value as an immunogen and for the
purification of viral proteins.
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STORAGE AND
STABILITY: |
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purified virus is stable for at least one year when stored at -70°C.
To avoid freeze-thawing, unused material should be stored in
aliquots, preferably under nitrogen in order to prevent oxidation.
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AVAILABILITY: |
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Distributed in vials containing 1.0 mg of viral protein, shipped on
dry ice. Protein concentration ranges from 0.5 to 1.5 mg/ml. Custom
orders are available, including (1) specific buffer formulations,
(2) package sizes, (3) solid-phase adsorption to agarose, plastic,
etc. and (4) conjugation to fluorochromes (FITC, rhodamine, etc.),
enzymes, biotin, streptavidin, etc.
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CATALOG NUMBER
0810028 SIZE
1.0 mg
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(main)
(sales)
(contact us)
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872
Main Street, Buffalo, NY 14202
Phone: 800-274-5487
Fax: 716-882-0959 |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45