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This
Package Insert is provided for product evaluation purposes only and is
not intended to be used in place of the Package Insert shipped with the
product.
OXI-TEK
NUCLEOSIDE STANDARD
8-Oxo-dA
The development of this
product was supported in part by
grants from the National Cancer Institute (R42 CA80451)
and the New York Center for Biotechnology.
FOR RESEARCH USE ONLY. NOT FOR in vitro DIAGNOSTIC OR CLINICAL
USE
8-Oxo-2'-Deoxy Adenosine
(8-Oxo-dA)
(8-Oxo-dA): 8-Oxo-7,8
dihydro-2'-deoxyadenosine (Also known as 8-hydroxy- 2'-deoxyadenosine;
8-oxo-2-deoxyadenosine)
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PRODUCT
CHARACTERISTICS |
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(8-Oxo-dA) serves as
a marker of oxidative DNA damage in biological systems.(1)
(8-Oxo-dA) has been chemically synthesized to improve
reliability and accuracy and purified by HPLC. (2)
Identification of the product and an index of its purity was
accomplished by proton NMR spectroscopy at 400 MHz.(3) |
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RECOMMENDED
USAGE |
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8-Oxo-dA may be used
as an internal standard for quantitation of DNA damage by
various methods including LC-MS. |
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FORMULA/WEIGHT/
PURITY |
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Mol. F:
C10H13N5O5 FW: 283.24 Purity: >98% |
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STORAGE
AND STABILITY |
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Supplied at ~1.0
mg/vial. Lyophilized product is stable for 1 year when stored at
2-8ºC. Recommend aliquoting and storing at -20°C when
resuspended in water. |
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NMR
SPECTRUM |
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REFERENCES |
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(1)
A. Weimann, D. Belling, H.E. Poulsen, Free Radic. Biol. Med.
(2001) 30, 757-764.
(2) A.E. Maccubbin, H.B. Patrzyc, N. Ersing, Budzinski, J.B.
Dawidzik, J.C. Wallace, H. Iijima, H. C. Box,
Biochimica et Biophysica Acta (1999) 1454, 80-88.
(3) H.C. Box, H.G. Freund, E.E. Budzinski, J.C. Wallace and
A.E. Maccubbin,
Radiation Research, (1995) 141, 91-94. |
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