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This
Package Insert is provided for product evaluation purposes only and is
not intended to be used in place of the Package Insert shipped with the
product.
OXI-TEK
DINUCLEOTIDE STANDARD
Thymine Glycol Adenine Dimer d(TgpA)
The development of this
product was supported in part by
grants from the National Cancer Institute (R42 CA80451)
and the New York Center for Biotechnology.
FOR RESEARCH USE ONLY. NOT FOR in vitro DIAGNOSTIC OR CLINICAL
USE
Thymine
Glycol Adenine Dimer d(TgpA)
d(TgpA):
Dinucleotide Monophosphate where Tg
is cis-5R, 6S-thymidine glycol.(1,
2)
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PRODUCT
CHARACTERISTICS |
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d(TgpA)
is useful as an internal standard for end-labelling as well as
LC-MS analysis in the investigation of the nature and
consequences of DNA damage. d(TgpA)
has been chemically synthesized to improve reliability and
accuracy and purified by HPLC.(3)
Identification of the product and an index of its purity was
accomplished by proton NMR spectroscopy at 400 MHz.(4) |
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RECOMMENDED
USAGE |
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d(TgpA)
may be used as an internal standard for various quantitations.
This lyophilized product is supplied at 1.0 ODU ( ~50 µg) as a
mixture of the cis isomers. Reconstitute in water to 1.0
ml. |
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STORAGE
AND STABILITY |
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Lyophilized product is stable for 1 year when stored at 2-8ºC.
Recommend aliquoting and storing at -20°C when resuspended in
water. |
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NMR
SPECTRUM |
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REFERENCES |
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(1) E. Schroder, E.E. Budzinski, J.C. Wallace, J.D. Zimbrick
and H.C. Box, International Journal of
Radiation Biology (1995) 68, 509-523.
(2) J.D. Baleja, G.W. Buchko, M. Weinfeld and B.D. Sykes,
Journal of Biomolecular
Structure and Dynamics (1993) 10, 747-762.
(3) A.E. Maccubbin, H.B. Patrzyc, N. Ersing, Budzinski, J.B.
Dawidzik, J.C. Wallace, H. Iijima, H. C.
Box, Biochimica et Biophysica Acta 1454 (1999) 80-88.
(4) H.C. Box, H.G. Freund, E.E. Budzinski, J.C. Wallace and
A.E. Maccubbin,
Radiation Research, 141. 91-94 (1995) |
Rev.:01/03 |
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