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This Package Insert is
provided for product evaluation purposes only and is
not intended to be used in place of the Package Insert shipped with the
product.
OXI-TEK
DINUCLEOTIDE STANDARD
Formamido Thymine Dimer d(PfpT)
The
development of this product was supported in part by
grants from the National Cancer Institute (R42 CA80451)
and the New York Center for Biotechnology.
FOR RESEARCH USE ONLY. NOT
FOR in vitro DIAGNOSTIC OR CLINICAL USE
Formamido Thymine
Dimer d(PfpT)
d(PfpT): Dinucleotide monophosphate
where Pf is the nucleoside bearing
the 1´-formamido-2´deoxy-ß-D-ribose modification from a pyrimidine
nucleoside.
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PRODUCT
CHARACTERISTICS |
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d(PfpT) is useful as an internal
standard for end-labelling as well as LC-MS analysis in the
investigation of the nature and consequences of DNA damage. d(PfpT)
has been chemically synthesized to improve reliability and
accuracy and purified by HPLC.(1)
This modified monophosphate is derived by oxidation of
pyrimidines. Identification of products and an index of their
purity was accomplished by proton NMR spectroscopy at 400 MHz.(2) |
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RECOMMENDED
USAGE |
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d(PfpT)
may be used as an internal standard for various quantitations.
This lyophilized product is supplied at 1.0 ODU (~50 µg).
Reconstitute in water to 1.0 ml |
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STORAGE
AND STABILITY |
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Lyophilized product
is stable for 1 year when stored at 2-8ºC. Recommend aliquoting
and storing at -20ºC when resuspended in water. |
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NMR
SPECTRUM |
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REFERENCES |
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(1) A.E.
Maccubbin, H.B. Patrzyc, N. Ersing, Budzinski, J.B. Dawidzik,
J.C. Wallace, H.
Iijima, H. C. Box, Biochimica et Biophysica Acta 1454 (1999)
80-88.
(2) H.C. Box, H.G. Freund, E.E. Budzinski, J.C. Wallace and
A.E. Maccubbin,
Radiation Research, 141. 91-94 (1995) |
Rev.:01/03 |
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