|
This
Package Insert is provided for product evaluation purposes only and is
not intended to be used in place of the Package Insert shipped with the
product.
OXI-TEK
DINUCLEOTIDE STANDARD
Formamido Guanine Dimer d(PfpG)
The development of
this product was supported in part by
grants from the National Cancer Institute (R42 CA80451)
and the New York Center for Biotechnology.
FOR RESEARCH USE ONLY. NOT FOR in vitro DIAGNOSTIC OR CLINICAL
USE
Formamido Guanine
Dimer d(P fpG)
d(P fpG):
Dinucleotide monophosphate where Pf
is the nucleoside bearing
the 1׳-formamido-2׳deoxy-ß-D-ribose modification from a pyrimidine
nucleoside.
| |
|
|
PRODUCT
CHARACTERISTICS |
|
d(PfpG)
is useful as an internal standard for end-labelling as well as
LC-MS analysis in the investigation of the nature and
consequences of DNA damage. d(PfpG) has been chemically
synthesized to improve reliability and accuracy and purified by
HPLC.(1)
This modified monophosphate is derived by oxidation of
pyrimidines. Identification of the product and an index of its
purity was accomplished by proton NMR spectroscopy at 400 MHz.(2) |
| |
|
|
RECOMMENDED
USAGE |
|
d(PfpG)
may be used as an internal standard for various quantitations.
This lyophilized product is supplied at 1.0 ODU (~50 µg).
Reconstitute in water to 1.0 ml. |
| |
|
|
STORAGE
AND STABILITY |
|
Lyophilized product
is stable for 1 year when stored at 2-8ºC. Recommend aliquoting
and storing at -20°C when resuspended in water. |
| |
|
|
NMR
SPECTRUM |
|
 |
| |
|
|
|
REFERENCES |
|
(1) A.E. Maccubbin, H.B. Patrzyc, N. Ersing, Budzinski, J.B.
Dawidzik, J.C. Wallace, H.
Iijima, H. C. Box, Biochimica et Biophysica Acta 1454
(1999) 80-88.
(2) H.C. Box, H.G. Freund, E.E. Budzinski, J.C. Wallace and
A.E. Maccubbin,
Radiation Research, 141. 91-94 (1995) |
Rev.: 01/03 |
ZeptoMetrix
Corporation
872 Main Street
Buffalo, New York 14202
Office Phone: 716-882-0920
Fax: 716-882-0959
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45