ZeptoBlock is a proprietary immunoassay reagent formulated to block nonspecific binding sites of microwell plates to which antigen or antibody has been applied, thus minimizing interfering substances and cross reactivity. The reagent is supplied in a liquid, ready-to-use format.

To optimize the assay, ZeptoBlock is applied to block nonspecific binding sites on microwell plates after antigen or antibody has been bound to the plates. Although other compounds including whole serum, albumin, casein or gelatin can be used as blocking agents, ZeptoBlock is specifically formulated for this purpose. Add 200 µl of ZeptoBlock to each microwell and incubate for 2 hours at room temperature. Aspirate the remaining solution. Store plate at 4°C in a resealable bag with a desiccant.

Although ZeptoBlock may be used alone, to optimize stability of the prepared plates, we recommend using an immunoassay stabilizing reagent such as ZeptoCoat. After aspirating ZeptoBlock from the plate, add 100 µl of ZeptoCoat to each well. Incubate for 1 hour at room temperature. Aspirate the remaining solution and allow the plate to dry for a minimum of 4 hours prior to sealing in a bag with a desiccant. Store at 4°C.

Store at 4°C. ZeptoBlock contains no preservatives. If reagent is not used within 7 days after opening, use aseptic technique when removing contents.