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This Package
Insert is provided for product evaluation purposes only and is
not intended to be used in place of the Package Insert shipped with
the product.
ZeptoBind
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ZMC
Catalog # : 0801184L |
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Unit
Size: 1000 ml |
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ZMC
Catalog # : 0801184 |
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Unit
Size: 60 ml |
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PRODUCT
CHARACTERISTICS |
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Preparation of
microwell plates involve binding the antigen or antibody to
the plate, blocking nonspecific binding sites, then coating
the plate with a stabilizing reagent to allow for dry
storage. ZeptoBind is an immunoassay stabilizing reagent,
specifically formulated to aid in the adherence of antigens
or antibodies to microwell plates. When used properly,
ZeptoBind ensures binding of the protein matrices to the
plates. The reagent is supplied in a liquid, ready-to-use
format. |
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RECOMMENDED
USAGE |
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Dissolve the
antigen or antibody in ZeptoBind. Add 100 µl of the
suspension to each microwell and incubate overnight at 4°C.
Aspirate the remaining solution. The concentration of
antibody or antigen will greatly affect the quality of the
assay being developed. We recommend titrating the amount of
antigen or antibody to be used for coating the wells. For
most antibodies or antigens, the optimal coating
concentration is in the range of 1 to 10 µg/ml when using
ZeptoBind. If the optimal concentration is unknown and it is
not practical to perform a titration to determine this
concentration, we recommend using 10 µg/ml which works well
for most applications. A complete discussion of optimization
procedures can be found in Harlow and Lane, "Antibodies,
A Laboratory Manual", Cold Spring Harbor Laboratory,
1988.
It is
recommended that after binding the antibody or antigen to
the plate, that a blocking reagent such as ZeptoBlock and a
stabilizing reagent such as ZeptoCoat are applied. After
binding the antigen or antibody to the plate, add 200 µl of
ZeptoBlock to each well. Incubate for 2 hours at room
temperature. Aspirate remaining solution then add 100 µl of
ZeptoCoat to each well. Incubate for 1 hour at room
temperature. Aspirate the remaining solution. Dry the plate
for a minimum of 4 hours at room temperature prior to
placing in a plastic bag with a desiccant. Store at 4°C. |
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STORAGE
AND STABILITY |
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Store at 4° C. ZeptoBind contains no preservatives. Use
aseptic technique when removing contents. |
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Rev. 12/00
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45