To optimize the assay, ZeptoCoat is applied to stabilize adsorbed or immobilized proteins after the matrices have been bound to the plate and a blocking solution has been applied. Add 100 µL of ZeptoCoat to each well and incubate for 1 hour at room temperature. Aspirate the remaining solution and allow the plate to dry for a minimum of 4 hours at room temperature. To optimize stability, store the plate at 4°C in a resealable bag with a desiccant.

ZeptoCoat may be used alone or in conjunction with a blocking solution. We recommend using a blocking agent to eliminate nonspecific binding of protein to the plate and minimize background. Casein, gelatin, serum or albumin may be used or ZeptoBlock which is specifically formulated for this purpose. Prior to coating the plate with ZeptoCoat, add 200 µl of ZeptoBlock to each well and incubate at room temperature for 2 hours. Aspirate the remaining solution and add 100 µl of ZeptoCoat to each well. Aspirate the remaining solution and allow the plate to dry at room temperature for a minimum of 4 hours prior to sealing in a bag with a desiccant. Store at 4°C.

Store at 4°C. ZeptoCoat contains no preservatives. If reagent is not used within 7 days after opening, use aseptic technique when removing contents.