Two-colour labelling & detection system

The kit is intended for use with the 2-pad FAST® Slides, including the Serum Biomarker Chip. The kit contains the Universal Linkage System™ (ULS™) chemistry to label samples containing approximately 250 µg of protein in serum, plasma, or a whole cell lysate. The kit is designed to label two different protein samples, each with a different hapten. Sufficient labelling reagent is provided to perform a hapten swapping experiment.

> Two-Colour Labelling & Fluorescent Detection

Features and Benefits
 

• Highly efficient and uniform labelling of complex serum samples
• Reproducible labelling and signal detection
• Stable, robust and fast non-enzymatic procedure
• Reduces pH dependency of labelling efficiency
• System solution includes labelling reagents, fluorescent conjugate and bench-friendly protocol
• Accounts for hapten-specific differences in either Biotin-ULS™ or Fluorescein-ULS™ labelling efficiencies
• Averages differences in antibody-antigen binding interactions caused by steric hindrance
• Minimizes chip-to-chip variability - includes an internal control within the assay

The first pad on the slide is probed with a mixture of two different protein samples, each labelled with a different hapten; the second pad is probed with the same two protein samples but with the haptens reversed. The normalized intensity for each element of each pad is calculated as the average of the biotin- and fluorescein-labelled derived intensities from a two-pad experiment. The ratio between the signal intensity at each spot corresponds to the concentration ratio of the proteins found in the two samples. This method is attractive for antibody chips as it takes into account any hapten-specific differences in antigen-antibody interactions.

The use of the ULS labelling system minimizes background by using indirect fluorescence detection, labels multiple amino acids, and requires no additional materials or reagents.