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Solid phase extraction (SPE)

 

 

Solid phase extraction (SPE)
> Solid phase extraction columns
Whatman SPE devices are designed to concentrate or isolate analytes from complex sample matrices. Available with a variety of packing media, these devices offer the advantage of working with different types of interactions between the sample components, sorbent and suitable eluent. The polarity (polar, nonpolar) or charge (anion, cation) of the analyte of interest will determine the proper choice of sorbent and solvent. 

Whatman SPE devices have silica-based chemistries and are available in several configurations. Column capacities include 3, 6 and 12 mL size columns. A cartridge format for use with a syringe is also available.

 

Solid phase extraction is a chromatographic technique used to prepare samples for subsequent analysis by removing interfering substances that may be present. This is done either by retaining the substance of interest and washing off everything else or by retaining the interfering substances and eluting the product of interest.

Whatman SPE devices contain high quality sorbets for LC chromatographers.

Features and Benefits

  • Available in a range of packing media
  • Whatman quality sorbents for consistent results

 

Applications

  • Isolate analytes from complex sample matrices
  • Remove interfering substances in order to prepare samples for subsequent analysis
  • Drug metabolites in biological fluids
  • Food analysis
  • Environmental analysis

 

Solid Phase Extraction (SPE) Disks

The Whatman SPE disk utilizes C-18 derivatized silica, incorporated into a glass microfiber matrix. The high flow and high loading capacity of the glass microfiber media allow for rapid aqueous sample flow rates, while oil, grease and other organic analytes are efficiently extracted and retained by the reverse phase silica material.

Features and Benefits

  • High quality Whatman glass microfiber media for superior flow and high loading capacity
  • Efficient grease analyte extraction and retention

 

Applications

  • Oil and grease analysis, EPA Method 1664A

 

Sample Drying Device

A sample drying device is available for removal of water from organic extracts. It attaches to the male luer outlet of a syringe in which the organic sample extract has been collected. Traces of water are removed as the sample is pushed through the drying device into a collection vial.

 

Typical Data
Key to Sorbent Abbreviations Features
ODS-4 Octadecyl silane 14% of carbon load, end capped
ODS-5 Octadecyl silane 18% of carbon load, end capped
C-8 Octyl silane 8.5% of carbon load, end capped
FLO Florisil®
SCX Strong cation exchanger–  
  aromatic benzene sulfonic acid
  functional groups  
SAX Strong anion exchanger–  
  quaternary amino groups (-NR3+)

 

 

Ordering Information + Solid Phase Extraction
Catalogue Number Product Code Column Volume Quantity/Pack
Column Type
6803-0505 ODS-5 500 mg/3 mL 50
6803-0507 ODS-5 500 mg/6 mL 30
6803-0509 ODS-5 1000 mg/12 mL 20
6803-1205 C-8 500 mg/3 mL 50
6803-1809 FLO 1000 mg/12 mL 20
6803-2005 SAX 500 mg/3 mL 50
6803-2605 SCX 500 mg/3 mL 50
Cartridge Type*
6804-0405 ODS-4 500 mg/unit 50
6804-0505 ODS-5 500 mg/unit 50

 

 

Ordering Information - Solid Phase Extraction (SPE) Disks
Catalogue Number Description Filter Media Diameter (mm) Pore Size (µm) Quantity/Box
6805-3402 SPE disk for oil and grease 47 20
6805-3043 SPE disk for oil and grease 47 80
6805-3048 SPE disk for oil and grease 90 20
6805-8034 DFP prefilter 42.5 5 48
6805-8035 DFP prefilter for 47 mm disk PP 47 5 48
6805-8037 DFP prefilter for 90 mm disk PP 90 5 16

 

Ordering Information - Sample Drying Device
Cartridge Type with Polypropylene Filter
Catalogue Number Product Size (mg) Quantity/Pack
6805-8020 Sodium sulfate with 0.45 µm - PP filter and tube tip 1500 50
 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07