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Whatman protein array reagents have been optimised for use on FAST®
Slides. These reagents include protein arraying buffer, protein array
blocking buffer and protein array wash buffer.
Protein Arraying Buffer
Enhances long-term protein stability and molecular recognition
activity
Enhances fluorescent signal from arrayed sample
Optimized for use on FAST® Slides
Protein Array Blocking Buffer
Demonstrates highly efficient blocking of protein microarrays
Exhibits strong reduction of nonspecific antibody-antibody
interactions
Exhibits minimal effects on specific antibody-antigen interactions
Results in superior signal-to-noise ratios in protein microarray
applications
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| > Blocking with TBS
Tween 20, 0.1% - unspecific binding of biotinylated antibodies |
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Superior Blocking Capabilities
Reduction of nonspecific protein-protein interaction: Monoclonal
capture antibodies were arrayed on FAST Slides and probed with an
antibody cocktail consisting of 16 biotinylated polyclonal antibodies
followed by Streptavidin-Cy™5 detection. Images were taken at identical
laser/PMT setting. The first row of the array field is a positive
detection control.
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| > Blocking with Protein
Array Blocking Buffer |
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Blocking with Protein Array Blocking Buffer: Reduction of unspecific
antibody-antibody interactions by the protein array blocking buffer.
Protein Array Wash Buffer
Ideal washing buffer for protein microarrays
Conveniently supplied as 10x concentrate
Optimized for use on FAST Slides
The Protein Array Wash Buffer has been optimized for efficient
washing of protein microarrays to ensure optimum results. This buffer is
used as the washing buffer in FAST PAK, FAST Quant, and the Serum
Biomarker Chip kits.
| Ordering Information -
Protein Array Buffers & Reagents |
| Description |
Quantity/Pack |
Catalogue Number |
| Protein Arraying Buffer (2X) |
4 x 10 mL |
10 485 331 |
| Protein Arraying Blocking Buffer (1X) |
1 x 100 mL |
10 485 356 |
| Protein Array Wash Buffer (10X) |
4 x 125 mL |
10 485 330 |
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