PepChip Array

> Protein Kinase Targets in Your Hand

Multiplex Kinase Profiling with the PepChip® Kinase Array

A comparison of kinase patterns between normal and disease state or untreated vs. treated cells can be obtained by using the PepChip Kinase Array. It is also a useful tool to investigate the specificity of a kinase inhibitor in the context of the total kinase activity of the target cells using cell lysates.

Substrate Determination with PepChip® Kinase

Substrates for novel and known kinases are often determined using kinase assays. Traditionally, kinase assays are performed with only a few potential peptide or protein substrates. With PepChip Kinase you can screen 1,152 unique substrates in one experiment.

Synthetic Peptides Ensure Array-to-array Consistency

The PepChip Kinase array was designed for drug development and signal transduction research. The array consists of 1,152 synthetic kinase substrate peptides covalently bound to the surface of a treated glass slide. Synthetic peptides are highly stable and very consistent in structure, eliminating the problems that result when traditional protein array content is used.

Features and Benefits
 

• 1,152 peptide kinase substrates, derived from a large phosphoprotein database, on a single chip for fast determination of kinase substrate specificity at a low cost per data point
• 50 microliter total reaction volume for minimal use of valuable kinase protein sample per assay
• Peptide substrates covalently coupled to polymeric matrix for excellent substrate accessibility
• Compatible with isotopic and non-isotopic detection for high sensitivity and low background

PepChip Kinase

> PepChip Inhibition Activation

One million serum-starved cultured cells were left untreated (left panel), incubated with growth factor (middle panel) or incubated with growth factor and a specific small molecule tyrosine kinase inhibitor (right panel). After a 10 minute incubation, lysates were prepared and cellular kinome patterns were determined using PepChip Kinase microarrays. Statistical analysis showed that phosphorylation of 7% of the substrates changed significantly as a result of the treatment.

Layout of the PepChip Kinase Slides

The PepChip Kinase Slide contains peptides arrayed on a 25 x 75 mm glass slide. The array design contains two identical arrays of 1,176 peptides, including one rhodamine-labeled peptide per subarray used for quality control purposes, arrayed in a pattern of 4 x 6 subarrays. The PepChip Kinase Evaluation Slide contains two identical arrays of 192 peptides in a pattern of 2 x 4 subarrays containing 24 peptides. Test slides are available for optimization of reaction conditions.

Test slides available for optimization of reaction conditions

The PepChip workbook contains the listing of peptides on the PepChip Kinase Array ranked from the upper left to the lower right spot. Paste quantification results into this file for analysis. Click on the link below to download the PepChip Kinase Analysis Workbook.