Membrane accessories

Whatman offers the following membrane accessories to facilitate the testing processes of your laboratory:

1. Apparatus pressure filtration
2. Apparatus vacuum filtration
3. Membrane holders and accessories
4. Membrane prefilters

Apparatus pressure filtration
Batch filtration – Selectron®
Whatman offers a line of pressure filtration apparatus for clarification and filtration of aqueous liquids and gases.

> MD 050/0

Pressure filtration apparatus without sample funnels are for in-line use and are suitable for larger volumes of several liters. Apparatus with sample funnels are suitable for the batch filtration of volumes above 20 mL.

> MD 050/4

Liquid and gas filtration are possible; they can also be used for the sterile filtration of sera or the clarification of difficult-to-filter media, very viscous liquids in particular.

> MD 142/6/3

Membrane, paper or glass fibre filters can be used. Cleaning and changing filters are carried out with only a few actions. All units are provided with pressure-resistant filter supports. High-quality silicone or PTFE O-rings are used to seal the systems. For safety reasons please take care that the seals are not damaged. In addition to the stainless steel apparatus we offer PTFE versions for aggressive media.

> ML 050/0

Sampling and In-line Use – Selectron
The filtration apparatus are suitable for repeated use with membrane, paper or glass fibre circle filters.

> FP 050/1

They are particularly easy to take apart for cleaning or changing filters. All units are fitted with a pressure-resistant filter support. High quality silicone or PTFE seals ensure that the units do not leak. For safety reasons please take care that these sealing elements are not damaged. The filtration apparatus is made of stainless steel or polysulfone.

Membrane holders and accessories
Whatman offers a choice of holders for use with membrane filters.

> Membrane Filter Holders

Vacuum Type Glass Holders

Produced from borosilicate glass and available with a choice of support screen. Suitable for aqueous and organic solvent filtration. The funnel seal ensures that the sample does not bypass the membrane and that particulates are retained on the surface of the membrane.

The sintered glass support is recommended for filtration and biological analysis. The 304 stainless steel support screen is suitable for use with proteinaceous solutions.

Syringe Filter Type Membrane Filter Holders
 

Available in stainless steel and polypropylene with luer fittings for use with a standard syringe. The holders are designed for the quick and easy clarification, sterilization and removal of particulates from small volume samples, typically for HPLC applications. The holders contain PTFE gaskets and O-rings and allow the membrane to be autoclaved in place without the filter sticking to the holder.

Hardware/Replacement Parts

Whatman offers both stoppers and glass reservoirs.

Polyester Drain Discs

For use with membrane hardware where extra support is needed for improved flow rate and throughput. The Polyester Drain Disc is binder-free and has a thickness of 100 µm. It provides a flat surface to eliminate filter tearing or rupturing. It is also used as a separator between membrane layers in serial stack filtration applications. This chemically inert support disc is available in a variety of diameters for use in a range of devices.

Applications
 

Tissue culture media filtration

 

Typical Data
Filter Diameter (mm)	Membrane Holder
Membrane	Filter Systems—Glass	Reservoir Volume (mL)	Filter Surface Area (cm2)	Prefilter Diameter (mm)
25	FG 25	25	2.1	16
25	FG 25R	50	2.1	13
25	FG 25S	25	2.1	16
47	FG 47	300	9.6	35
47	FG 47S	300	9.6	35
90	FG 90	1000	38.5	70

 

Ordering Information - Membrane Holders and Accessories
Diameter (mm)	Description	Catalogue Number	Quantity/Pack
Membrane Filter Holders
25	Glass Support 50 mL–FG 25R Sintered Glass	1960-032	1
25	Glass Support 25 mL–FG 25 Sintered Glass	1960-002	1
25	Stainless Steel Support 25 mL FG 25S 304 Stainless Steel 100 Mesh Screen	1960-052	1
47	Glass Support 300 mL–FG 47 Sintered Glass	1960-004	1
47	Stainless Steel Support 300 mL FG 47S 304 Stainless Steel 100 Mesh Screen	1960-054	1
90	Glass Support 1000 mL–FG 90 Sintered Glass	1960-009	1
Hardware/Replacement Parts
	Stopper, adapts 25 mm funnel to manifolds, No. 8B	1960-500	1
	Glass reservoir for FG47 (300 mL)	1961-054	1
Accessories
10	Polyester Drain Disc	230300	100
22	Polyester Drain Disc	230500	100
25	Polyester Drain Disc	230600	100
37	Polyester Drain Disc	230800	100
47	Polyester Drain Disc	231100	100

Note: 25 mm holders have No. 5 stopper, fitting 125 mL flasks; 47 mm and 90 mm holders have No. 8 stoppers, fitting standard manifolds and 1 L flasks.
 

 

> Syringe Type Holders

Syringe Type Holders S/S

Luer lock fittings connect to a standard syringe and offer convenience and ease of use for clarification, sterilization and removal of particulates from small volumes of liquid (e.g., HPLC samples and solvents). PTFE gaskets and O-ring permit autoclaving of the membrane in place without the filter sticking to the holder.

 

 

 

> Plastic Filtration Holders

Plastic Filtration Holders

Pop-Top™ and Swin-Lok™ Plastic Filter Holders

Features and Benefits
 

• Designed for microfiltration and ultra cleaning of small volumes of liquids using positive pressure
• All three holders will accommodate Nuclepore track-etched and cast membranes
• Syringe compatible

 

Typical Data
Materials	13 mm Pop-Top	25 mm Swin-Lok	47 mm Swin-Lok
Holder	Polycarbonate	Polypropylene	Polycarbonate
Maximum Operating Temperature and Pressure	38°C (100°F) at 50 psi (3.5 bar)
Sterilization	121°C (250°F) for 15 minutes
Size (cm)	2.7 OD x 2.7 H	3.5 OD x 3.7 H	6.0 OD x 6.5 H
Membrane Size (mm)	13	25	47
Prefilter Size (mm)	10	22	42
Filtration Area (cm2)	0.8	3.9	13.8
Connection
Cap	Male luer slip-fit	Female luer-lok	Female luer slip-fit with Male 1/4" NPT and 1/4" Tubing (multi purpose)
Base	Female luer slip-fit	Male luer slip-fit

 

Ordering Information - Syringe Type Holders S/S
Filter Diameter (mm)	Description	Catalogue Number	Model	Prefilter Diameter (mm)
13	S/S, female luer inlet; male luer nozzle outlet	1980-001	SH13	10
25	S/S, female luer inlet; male luer nozzle outlet	1980-002	SH25	22

 

Ordering Information - Plastic Filtration Holders
Catalogue Number	Description	Diameter (mm)	Quantity/Pack
420100	Pop-Top	13	10
420200	Swin-Lok Holder	25	10
420400	Swin-Lok Holder	47	10

 

Membrane prefilters
The life of a membrane filter can be extended many times by placing a prefilter adjacent to or upstream of the membrane. The total particulate load challenging the membrane is considerably reduced thus allowing the membrane to operate efficiently.
 

Whatman manufacturers glass microfibres which are used as prefilters for membranes. The unique properties of borosilicate glass microfibres enable Whatman to manufacture filters with high loading capacity and retention of very fine particulates.

The Whatman Multigrade GMF 150, used as a prefilter, nearly doubles the volume of sample filtered compared to a single density prefilter. Compared to an unprotected membrane, the volume of sample filtered is three to seven times greater. Conventional prefilters cannot perform up to the same calibre as the Multigrade GMF 150 simply because prefilters of a uniform density do not have the loading capacity of the new multiporosity filter technology advanced by Whatman.