FAST Slides

FAST™ Slides are glass slides coated with a proprietary nitrocellulose polymer. The polymer binds proteins in a non-covalent, irreversible manner and can be probed using the same method as in traditional blots.

The three-dimensional surface of FAST Slide maintains reactivity of proteins and gives excellent reproducible results. It is usable with fluorescent, chemiluminescent or radiographic detection systems and is compatible with microarray scanners and robots.

Perhaps the most significant advantage of FAST Slides over modified glass surfaces is that the matrix retains arrayed proteins in near-quantitative fashion. This property translates into antibody arrays with unparalleled sensitivity down to antigen concentrations of 1 pg/ml. These qualities make the FAST Slides the most reliable surface for microarray experiments and provide a level of confidence that no other surface has.

Features and Benefits
 

• Superior protein binding capacity
• Highest sensitivity and dynamic range
• Excellent long-term stability of printed proteins
• Compatible with all detection methodologies
• Compatible with commercially available arraying robots

FAST Slides are suitable for many types of protein microarrays including antibody arrays and microarray Westerns. There are tremendous advantages to using FAST Slides over traditional ELISAs and Westerns including less sample required, better sensitivity, linearity and quantitation. The largest advantage is hundreds or thousands of antibodies or samples can be screened simultaneously.

Microarray Westerns

An alternative strategy for Protein Microarrays is to array samples containing multiple proteins on the FAST Slide and probe with labelled antibody or set of antibodies. The advantage of the micro format is that extracts from various treatments and time points can be arrayed on the same slide. Once arrayed, the levels of multiple proteins can be measured and compared simultaneously.