|
MicroSpot ELISA for High-Throughput Multiplex Cytokine Quantification
FAST Quant represents a quantum leap forward in protein microarray
technology. With FAST Quant, a researcher can accurately determine the
concentration of several cytokines in dozens of biological samples
simultaneously, using familiar ELISA immunochemistry.
Built on FAST® technology, the high protein binding capacity surface
chemistry, FAST Quant combines the power of array technology with the
quantitative nature and high-throughput capabilities of traditional
ELISA. FAST Quant exhibits sensitivity and reproducibility better than
traditional ELISA.
Each FAST Quant kit contains 64 arrays of eight to ten monoclonal
antibodies with affinities for common human or mouse cytokines. The
antibodies are arrayed in a quantitative fashion in triplicate on each
array.
Using four 16-pad FAST Slides placed in a FAST Frame (sold
separately), FAST Quant offers an 8 x 12 cm footprint - the same as the
traditional microplate format. A standard curve can be generated by
creating a dilutional series from recombinant antigen mass standards.
Due to the solid-phase nature of a microspot assay, it is not necessary
to take duplicate measurements of each sample. The MicroSpot ELISA
reaction is entirely concentration dependent, unlike an ELISA where the
reaction is both concentration and volume dependent.
FAST Quant antibody arrays offer a variety of human and mouse menus.
All arrays come in a four-slide kit with recombinant antigens standard,
detection antibodies and processing buffers.
Data analysis is seamless using data reduction software, ArrayVision™
FAST, the cutting edge utility for protein array image analysis. The
software provides rapid data acquisition and comprehensive reports.
ArrayVision analyzes any .tif image from virtually all commercial
imaging instruments. The application provides standard curve data,
concentrations of unknowns, and percent coefficient of variation for
each analyte. FAST Quant is another clear example of Whatman commitment
to providing the scientific community with best solution for multiplex
cytokine measurements.
| FAST® Quant Human and Mouse
Menus |
FAST Quant Human Th1/Th2
Cytokines commonly associated with the Th1/Th2 immune response
system |
FAST Quant Mouse
Th1/Th2
Cytokines commonly associated with the Th1/Th2 immune response
system |
| IL-1β |
IL-5 |
IL-13 |
IL-1β |
IL-5 |
IL-13 |
| IL-2 |
IL-6 |
TNFα |
IL-2 |
IL-6 |
TNFα |
| IL-4 |
IL-10 |
IFNγ |
IL-4 |
IL-10 |
IFNγ |
| FAST Quant Human II |
FAST Quant Mouse II |
| IL-1β |
IL-10 |
- |
IL-1β |
IL-12p70 |
- |
| IL-2 |
IL-12p70 |
- |
IL-2 |
GM-CSF |
- |
| IL-4 |
GM-CSF |
- |
IL-4 |
RANTES |
- |
| IL-8 |
RANTES |
- |
IL-6 |
IFNγ |
- |
| IL-6 |
MCP-1 |
- |
IL-10 |
- |
- |
| FAST Quant Human
Angiogenesis |
FAST Quant Human Chemokine |
| PDGF-BB |
KGF |
- |
Eotaxin |
MCP-4 |
- |
| VEGF |
TIMP-1 |
- |
RANTES |
IL-8 |
- |
| FGF-b |
ICAM-1 |
- |
MCP-1 |
IP-10 |
- |
| Angiogenin |
Angiopoietin-2 |
- |
MCP-2 |
MIP-1α |
- |
| - |
- |
- |
MCP-3 |
- |
- |
| Ordering Information - FAST®
Quant |
| Description |
Catalogue Number |
| FAST Quant Human Th1/Th2 |
10 486 031 |
| FAST Quant Mouse Th1/Th2 |
10 486 061 |
| FAST Quant Human II |
10 486 060 |
| FAST Quant Mouse II |
10 486 062 |
| FAST Quant Human Angiogenesis |
10 486 063 |
| FAST Quant Human Chemokine |
10 486 064 |
| Each kit includes: |
Four 16-pad FAST Slides;
each pad is pre-arrayed with a panel of cytokine antibodies
(choice of six panels)
Four silicon 16-well chambers
Recombinant antigen standard cocktail for dilutional series
(standard cruve)
Biotinylated detection antibody cocktail
S&S Protein Array Buffer, Protein Array Wash Buffer and Protein
Array Blocking Buffer
|
| FAST Frame Slide Holder (4 slides) |
10 486 001 |
| Chip Clip™ Slide Holder (single slide) |
10 486 081 |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
