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Membrane-based Antibody Arrays
Whatman FAST Macro membrane-based antibody arrays are used to
simultaneously evaluate the relative abundance of 20 different cytokines
between different biological samples, such as disease state versus
normal state, using chemiluminescent detection.
The FAST Macro Human I array consists of 20 anti-cytokine antibodies
arrayed on Whatman Protran nitrocellulose (BA-83, 0.2 µm), a surface
which is well known for high protein binding capacity and excellent
signal-to-noise ratios.
Designed as a screening tool to compare relative expression levels of
cytokines/growth factors in different samples, two human panels and one
mouse panel are included. The FAST Macro kit includes either four or
eight arrayed membranes, FAST Macro Wash and Blocking Buffers, and a
biotinylated antibody cocktail. Arrays are visualized using
streptavidin/HRP-based chemiluminescent detection and x-ray film or a
phosphor imager.
FAST Macro is an inexpensive way to discover the power of multiplex
antibody arrays with sensitivity as low as 15 pg/ml that can be used
with serum, cell lysates and culture media samples. Results are
comparable to ELISA results. No special instrumentation or software is
required for detection and analysis.
Features and Benefits
- Requires as little as 650 µL of sample
- Array-to-array reproducibility
- Cytokines are arrayed in triplicate with 9 positive and 3
negative control spots per membrane
- Tested for cytokine specificity - virtually no cross reactivity
- Membrane is notched in one corner for orientation
- Cytokine spots are visible on the unprocessed membrane - inert
dye disappears during membrane wet-out
Samples as small as 400 µL can be processed using the optional S&S
Chip Clip™ in conjunction with an S&S dual-well incubation chamber and a
standard, untreated glass microscope slide.
| Ordering Information - FAST®
Macro |
| Membrane-Based Microarrays |
| Description |
Quantity/Pack |
Catalogue Number |
| FAST Macro Human I Kit (4 arrays) |
1 |
10 486 151 |
| FAST Macro Human I Kit (8 arrays) |
1 |
10 486 152 |
| Chip Clip™ Slide Holder |
1 |
10 486 081 |
| DualWell Incubation Chambers |
10 |
10 486 087 |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
