Blotting Devices

Minifold® I system
Superior 96- and 48-well manifolds for proteins & nucleic acids
Dot-, Spot- and Slot-Blot Array Systems
The Minifold I system consists of four basic components: sample well plate, filter support plate, vacuum plenum, and metal clamping plate. The sample well plate is available in three configurations for producing spots, dots, or slots.

The Minifold I system is compatible with multichannel pipettes. All three plates are interchangeable and can be purchased as an accessory plate in conjunction with a complete system. The tension on the clamping is adjustable, permitting use of a variety of blotting and filtration media.
 

> 96-Well dot-Blot Plate

Minifold I Dot-Blot System - 96-Well

Unique O-ring design - ensures discrete dot formation without leakage of samples by cross lateral flow.

Generates even, uniform dots which eliminates uneven test areas resulting from manual sample application.

Two materials available - standard acrylic or autoclavable Delrin® .
 

> 96-Well Spot-Blot Plate

Minifold I Spot-Blot System - 96-Well

Small volume required - sample volumes as low as 25 µL can be applied using less of your precious sample.

Very high signal intensity - 2 mm² sample application area results in increased signal intensity.

Standard microtitration format- 96 samples on a single membrane; same as the standard Minifold Dot-Blot system.
 

> 48-Well Spot-Blot Plate

Minifold I Slot-Blot System - 48-Well

Preferred format for densitometric scanning - slots can be easily quantitated.

Concentrated signal - 6 mm² sample application area results in high signal intensity.

Easy to survey format - 48 samples on your membrane are easier to view than 96 samples.
 

Delrin is a registered trademark of DuPont

Minifold® II system
 

72-Well Slot-Blot Array System
The Minifold II system is designed for precise quantitative solid-phase assays with three rows of 24 slots, spaced according to multi-tip pipette format. The smaller slot surface area results in higher signal intensity and requires less sample than standard dot-blot formats. The resulting blot can be read with a densitometer.

Features and Benefits
 

• Higher signal intensity - smaller slot surface area results in increased signal
• Less sample - small slot dimensions require less sample than dot-blotters
• Faster results - more intense signal generated with the slot-blotter allows to visualize results in less time
• Accepts all types of transfer membranes - allows for choice of membrane with the highest binding capacity
• Easy to assemble - beveled side rails assure rapid and accurate assembly

TurboBlotter™
Rapid Downward Transfer System
The TurboBlotter system is a rapid downward blotting device for high-resolution transfer of DNA and RNA.
 

The conventional Southern transfer stack has been turned upside down in order to take advantage of gravity. No heavy weights are required on the top of the transfer stack, eliminating the messy setup of standard upward capillary transfers.

The TurboBlotter system offers greater speed, target resolution and convenience versus traditional blotting procedures. Alkaline DNA transfers can be performed in as little as one hour, while neutral (SSC) transfers of DNA and RNA take only three hours.

Features and Benefits
 

• Rapid - unique downward capillary transfer allows for alkaline buffer transfers in one hour and neutral (SSC) transfers in three hours.
• Economical - reusable blotting device requires less buffer and blotting paper. Convenient refill packs available.
• Compact - has a smaller footprint than most homemade devices and is stackable. Up to 5 units can be stacked on top of each other during transfers.
• Easy to use - very easy to set up, and works without power or vacuum source

Each TurboBlotter system contains a transfer device, as well as the blotting paper and membranes for five transfers. The refill packs contain the blotting paper and membranes for five transfers.