Application Specific Plates

> Dye terminator removal UNIFILTER

They are constructed from rigid polystyrene that can withstand centrifugation. Laboratory packing of gel filtration media is less expensive than pre-packed plates or spin columns.

> ELISA UNIFILTER

Chemiluminescent assays can be read in the plate. If the ELISA product is eluted then centrifugation is preferred. This microplate is compatible with automated systems.

> High throughput genomics UNIFILTER

This microplate has a Cellulose Acetate membrane with a special support, which clears nonchaotropic bacterial lysates, and long drip directors. Without further purification, the DNA is clean enough for further enzymatic manipulation. Cellulose Acetate acts as both a depth filter and a fine particle filter. The 0.45 µm pores do not block because of the depth effect of the membrane. Cellulose Acetate is also inert and does not bind either DNA or protein.

> PCR cleanup UNIFILTER

The PCR Cleanup UNIFILTER can be used with both vacuum and centrifuge techniques. (Centrifugation is recommended for final elution with the 384 well UNIFILTER.)

> Microplates

Whatman 1PS is a silicone-treated medium which remains impervious to aqueous solvents but allows the unimpeded passage of organic solvents. Providing that the solvent layer is in contact with the 1PS, the organic solvent layer will drain under gravity until the aqueous interface is reached, when flow will stop automatically. If subsequent harvesting of the aqueous layer is required, a vacuum can then be applied to remove this layer.

> Protein kinase assay UNIFILTER

The filter plate is produced to SBS standards in rigid white polystyrene or Barex to eliminate optical crosstalk problems during Liquid Scintillation Counting. The 150 µL UNIFILTER has shallow wells enabling higher detection sensitivity.

> Protein precipitation UNIFILTER FF

The plates contain specially formulated dual membranes with two distinct layers. The top layer acts as a prefilter to remove coarse particulates. The bottom layer is oleophobic for retaining the well contents without dripping. This provides a final filter for removing fine particulate matter when a vacuum is applied. The plates are available in the following well bottom formats: Standard; Fast Flow; Round Bottom and "V" Bottom.

• 96 samples purified at the same time
• Purified samples available in less than 10 minutes
• Precipitation and filtration in the same well
• No laborious pipetting and/or centrifugation required and minimal liquid handling
• Dual Whatman filter media
• Ensures no fluid loss during incubation and fast flow during filtration
• Over 98% protein removal
• Sample volumes up to 150µL for plasma and 200µL for serum
• SBS compatible with the exception of the "V" bottom plate
• Robotics friendly

> 96 Well bacterial growth plate

Whatman has demonstrated that culture integrity is not affected by the close proximity of neighbouring cultures and that each culture grows to the same density as it would in individual culture tubes (at 325 rpm and 37°C for 16 hours).

• Consistent cell densities across all 96 wells
• Eliminates cross contamination between wells
• Growth comparable to individual test tubes
• Ability to automate allows for increased productivity

> 96 Well DNA binding UNIFILTER

Plasmid DNA is bound to the filter under chaotropic conditions, washed twice and then vacuumed to dryness on a vacuum manifold. The plasmid DNA is eluted by vacuum in a final volume of 100 µL into a nonbinding polypropylene collection plate using water or TE-1 Buffer. The DNA is ready to use and further ethanol precipitation is unnecessary. The final concentration is 50 to 100 ng/µL, depending on the original culture. The OD260/280 ratio is 1.9 and the yields in all 96 wells "max out" at 6 µg. Full protocol is available on request.

The Plasmid DNA Binding plate can be used with both vacuum and centrifuge techniques, making it a vital and flexible tool in every high throughput lab.

• DNA recovery of 6µg per well on average
• Consistent yield across all 96 wells
• Eluted plasmid DNA is free of genomic DNA contamination
• High quality DNA suitable for PCR, restriction digestion and sequencing
• Save time, no desalting or ethanol precipitation
• No kit required, significantly reduces costs

> 96 Well lysate clarification UNIFILTER

Whatman filter technology results in high particle retention and fast flow rates while producing a clean lysate. The Lysate Clarification plate is an important tool for high throughput plasmid DNA purification.

• Process 96 lysates in less than 10 minutes
• DNA recovery of 6µg per well on average
• Increase DNA recovery by 10 to 30%
• Consistent yield across all 96 wells
• User flexibility of using either centrifugation or vacuum
• 96 well format is easily automated

> Plasmid miniprep

The Whatman Plasmid Miniprep System consists of a few basic steps, each with an optimized microplate.

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

Send mail to webmaster with questions or comments about this web site.
Copyright © 2005 Gentaur BVBA
Last modified: feb-07