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TACS™ In Situ Apoptosis Detection Kits (TUNEL Assays)
TACS·XL® Kits
TACS™ 2 TdT Kits
TACS™ 1 and NeuroTACS 1
Klenow Kits
| TACS•XL®
embodies a new approach for the in situ detection of
apoptosis. The TACS•XL kit is based on incorporation of
bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments
that are formed during apoptosis. The incorporation of BrdUTP by
TdT is more efficient than either biotinylated or digoxigenin
labeled nucleotides used in other TUNEL-based assays. The
detection system utilizes a biotin conjugated anti-BrdU antibody
and streptavidin-horseradish peroxidase. The combination of
antibody specificity with the signal enhancing properties of
biotin:streptavidin results in precise cellular labeling and the
highest signal to noise ratio observed in competitive testing.
Three kit formats are available: TACS•XL DAB, TACS•XL Blue Label
and TACS•XL Basic. The TACS•XL DAB kit is supplied with DAB and
Methyl Green counterstain, and the TACS•XL Blue Label kit is
provided with TACS Blue Label and Nuclear Fast Red counterstain.
These complete kits provide all the reagents required for
labeling including two permeabilization reagents, labeling and
stop buffers, labeling and detection reagents, and TACS-Nuclease™
reagents for generating positive controls with your own samples.
The TACS•XL Basic kit provides the labeling reagents necessary
for routine labeling, and is ideal for researchers whose labs
are equipped for standard immunohistochemical procedures
involving horseradish-peroxidase. Please refer to our components
listing for details. |
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High signal to
noise ratio generates stronger signal with less
background. |
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Less sensitive
to protease-induced false positive labeling than
digoxigenin or biotin-based kits. |
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Complete kit
provides either DAB or TACS Blue Label detection
options. |
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Includes
exclusive Cytonin™
permeabilization reagent. |
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Includes
TACS-Nuclease control reagents. |
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Readily
adapted for fluorescence read-out. |
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DAB kit now
includes a DAB enhancer to intensify and darken DAB
staining! |
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In situ
detection of apoptosis in fixed frozen or paraffin
embedded samples. |
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Assists in the
identification of apoptotic morphologies. |
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| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables |
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| Components are stored at -20°C,
4°C, and room temperature. |
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Products
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4828-30-K |
TACS·XL® Basic Kit, 30 Samples |
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4828-30-BK |
TACS·XL® Blue Label Kit, 30 Samples |
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4828-30-DK |
TACS·XL® DAB Kit, 30 Samples |
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4828-30-R |
TACS·XL® Replenisher Kit, 30 Samples |
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4828-30-BC |
Blue Label Detection Module |
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4828-30-DC |
DAB Detection Module |
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4828-30-N |
Nuclease Module |
The TACS™
2 TdT Kits utilize Trevigen’s unique cation optimization system
to enhance labeling within particular tissues. The TACS™
2 TdT Kits employ a proprietary labeling buffer that contains no
toxic components (e.g. sodium cacodylate). A highly purified
form of the TdT enzyme is included in the kits for the enzymatic
incorporation of biotinylated nucleotides. Biotin labeling is
achieved using streptavidin-horseradish peroxidase, and
colorimetric substrates
 diaminobenzidine
(DAB) or TACS™ Blue Label™.
For fluorescent detection, a fluorescein conjugate of
streptavidin is used and visualized by epifluorescence
microscopy. Additional fluorophores are now available separately
from Trevigen.These complete kits provide all the reagents
required for labeling includingtwo permeabilization reagents,
labeling and stop buffers, labeling and detection reagents, and
TACS-Nuclease™ reagents for
generating positive controls with your own samples. Please refer
to our components listing for details.
The core kit is for researchers familiar with in situ
labeling who are already set up for immunohistochemistry. A
replenisher kit is also available for users of our TACS™
kits who have used up the limiting reagents within the kit. This
kit provides a cost effective replacement of the reagents that
are used most quickly.
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TdT based
in situ labeling. |
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Cation
optimization for high signal and low background. |
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Complete kit
provides either DAB, TACS™
Blue Label, or fluorescence detection options. |
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Includes
exclusive Cytonin™
permeabilization reagent. |
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Includes
TACS-Nuclease control reagents. |
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DAB kit now
includes a DAB enhancer to intensify and darken DAB
staining! |
|
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In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cells and tissues. |
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Light
microscopy |
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Fluorescence
microscopy |
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Flow cytometry |
|
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| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables |
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| Components are stored at -20°C,
4°C, and room temperature. |
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Products
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4810-30-K |
TACS™ 2 TdT DAB Kit, 30 Samples |
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4811-30-K |
TACS™ 2 TdT Blue Label Kit, 30 Samples |
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4812-30-K |
TACS™ 2 TdT Fluorescein Kit, 30 Samples |
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4810-30-CK |
TACS™ 2 TdT Core Kit, 30 Samples |
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4810-30-R |
TACS™ 2 TdT Replenisher Kit, 30 Samples |
| The TACS 1
Klenow kits and NeuroTACS I use a highly purified form of the
Klenow enzyme (large fragment of DNA polymerase I) for the
enzymatic incorporation of a biotinylated nucleotide. Detection
of biotin incorporation with the Klenow kits are visualized
using streptavidin-horseradish peroxidase. NeuroTACS I provides
diaminobenzamide (#4800-30-K) along with a Blue Counterstain for
a good contrast to the brown DAB precipitate. The TACS 1 kits
provide two options for colorimetric detection with
diaminobenzidine (Cat# 4800-30-K) or TACS Blue Label (Cat#
4800-30-BL). For fluorescent detection, a fluorescein conjugate
of streptavidin is used and visualized by epifluorescence
microscopy. The NeuroTACS kit provides reagents and protocols
optimized for the detection of apoptosis in neuronal tissues and
cell lines. A key feature of this kit is NeuroPore, a
proprietary permeabilization reagent that gently permeabilizes
samples while retaining cell morphology.
The TACS 1 core kit is for researchers familiar with in
situ labeling and are prepared to perform histochemistry.
It provides Apoptosis Grade Klenow and nucleotide mix, labeling
buffer, stop buffer, and streptavidin-horseradish peroxidase
conjugate, all of which have been performance tested for in situ
labeling. |
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Klenow based
in situ labeling. |
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TACS 1
provides DAB, TACS Blue Label, or fluorescence detection
options along with our exclusive Cytonin™
permeablization reagent. |
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NeuroTACS I
provides our proprietary NeuroPore permeabilization
reagent. |
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In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cells and tissues. |
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TACS 1 can be
used with light microscopy ,fluorescence microscopy, or
flow cytometry. |
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NeuroTACS I
helps resolve unique problems encountered when detecting
apoptotic neuronal cells. |
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| Store components at -20°C, 4°C, and
room temperature. |
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Products
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4800-30-K |
TACS™ 1
Klenow DAB Kit, 30 samples |
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4805-30-K |
TACS™ 1
Klenow Blue Label Kit, 30 samples |
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4806-30-K |
TACS™ 1
Klenow Fluorescein Kit, 30 samples |
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4800-30-CK |
TACS™ 1
Klenow-Core Kit, 30 samples |
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4800-30-R |
TACS™ 1
Klenow-Replenisher Kit ,30 samples |
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4820-30-K |
NeuroTACS™ I Kit,30 samples |
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