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Specialty In Situ Apoptosis Detection Kits (TUNEL Assays)
CardioTACS™ Kit
DermaTACS™ Kit
NeuroTACS™ II Kit
TumorTACS™ Kit
TiterTACS™ Kit
FlowTACS™ Kit
VasoTACS™ Kit
The CardioTACS™
Kit was developed to provide the heart researcher with an
effective method for identifying apoptotic cells in cardiac
samples. The high cellularity of cardiac tissue presents
problems in permeabilization, so the CardioTACS Kit comes with
two permeabilization reagents to provide options. The kit is
based on DNA end-labeling using terminal deoxynucleotidyl
transferase (TdT) and a modified nucleotide that is subsequently
detected using our TACS Blue Label™
detection system. Trevigen has developed an exclusive apoptosis
grade TdT enzyme that coupled with the TACS Blue Label solution
provides labeling 20 to 50 times more sensitive than standard
diaminobenzidine (DAB) labeling methods. To ensure ease of data
interpretation when the numbers of apoptotic cells are low,
CardioTACS provides Nuclear Fast Red Counterstain which provides
superb contrast to the TACS Blue Label in apoptotic cells. In
addition, the kit includes TACS-Nuclease™
that is used to generate a positive control in your own sample.
The positive control provides an internal control for
permeabilization and labeling so optimization is nominal and the
data can be interpreted with confidence.
These complete kits provides all the reagents required for
labeling including two permeabilization reagents, labeling and
stop buffers, labeling and detection reagents, TACS-Nuclease
reagent, and a protocol detailing incubation times and reagent
concentrations. |
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT buffer - sodium cacodylate free.
|
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Performance
tested on heart derived samples. |
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample dependent
positive controls. |
|
| |
| • |
In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cardiac cells and tissues. |
| • |
Assists in the
identification of apoptotic morphologies. |
| • |
Helps resolve
unique problems encountered when detecting apoptotic
cardiac cells. |
|
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
Counterstain |
| • |
Nucleotide mix |
| • |
Detection
reagents |
| • |
TACS-Nuclease |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4827-30-K |
CardioTACS™ Kit, 30 Samples |
DermaTACS™ Kit
The DermaTACS™
Kit was developed to provide researchers with an effective
method for measuring apoptosis in skin samples. The kit was
based on DNA end-labeling using terminal deoxynucleotidyl
transferase (TdT) and modified nucleotides. Detection of
incorporated molecules is achieved using a chromogenic substrate
with a horseradish peroxidase detection system. The
extracellular scaffolding in skin tissue can make it problematic
to permeabilize and high background is a common problem with
skin samples. The DermaTACS Kit includes two permeabilization
reagents as options for permeabilization along with a
 detailed
protocol with hints and tips for optimal labeling of skin
samples based on empirical testing. Low background is achieved
using a combination of the high specificity of Trevigen’s unique
cation based optimized TdT labeling reaction using Apoptosis
Grade™ TdT and TACS Blue Label™
as the visualization method. Also included is TACS-Nuclease™
to generate a positive control from your own sample. In
conjunction with the excellent contrast between the TACS Blue
Label and the Red Counterstain C, the positive control ensures
easy data interpretation.This complete kit provides all the
reagents required for labeling including two permeabilization
reagents, labeling and stop buffers, labeling and detection
reagents, and TACS-Nuclease. |
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Performance
tested on skin derived samples. |
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample-dependent
positive controls. |
|
| |
| • |
In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cells and tissues. |
| • |
Assists in the
identification of apoptotic morphologies. |
| • |
Helps resolve
unique problems encountered when detecting apoptosis in
skin sections. |
|
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
Counterstain |
| • |
Nucleotide mix |
| • |
Detection
reagents |
| • |
TACS-Nuclease |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4829-30-K |
DermaTACS™ Kit, 30 Samples |
|
4800-30-42 |
EpiDerm™ Control Slides, 2 slides |
NeuroTACS™ II Kit
NeuroTACS™
II is a complete reagent kit optimized to provide rapid and
convenient identification of apoptosis in brain tissue or
neuronal cells. The kit has been developed to overcome the
common difficulties unique to neuronal samples including the
fragile nature of brain tissue sections, high background
problems, poor counterstaining with common dyes, and the need to
perform dual labeling experiments to detect cell specific
antigens in conjunction with apoptotic cells. A key feature is
NeuroPore™, a proprietary
permeabilization reagent that gently permeabilizes samples while
retaining cell morphology. NeuroPore also contains blocking
reagents to allow its use as an antibody diluent in
 immunohistochemistry
and to reduce background staining. DNA fragments generated by
apoptosis are end-labeled with modified nucleotides using a
highly purified terminal deoxynucleotidyl transferase enzyme
(TdT). The incorporated nucleotides are detected using a
horseradish peroxidase system that catalyzes the conversion of
diaminobenzidine (DAB) into a dark brown precipitate. NeuroTACS
II contains the Blue Counterstain to allow visualization of all
cells within the sample with good contrast to the brown DAB
precipitate. In addition, the Blue Counterstain is compatible
with the red colored substrates used for phosphatase detection
in double labeling experiments. The protocol includes details
for labeling in situ for apoptosis and antigen
detection on the same sample. To ensure that your own samples
have been processed and permeabilized correctly, we provide
TACS-Nuclease™, a unique
reagent used to generate a positive control with your own
samples. This provides a high degree of confidence for data
interpretation and can help pinpoint problem steps in the
labeling procedure. |
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Performance
tested on brain sections. |
| • |
Includes
exclusive NeuroPore permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample-dependent
positive controls. |
| • |
Includes DAB
Enhancer for intensifying and darkening DAB staining. |
|
| |
| • |
In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cells and tissues. |
| • |
Assists in the
identification of apoptotic morphologies. |
| • |
Helps resolve
unique problems encountered when detecting apoptotic
neuronal cells. |
|
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
Counterstain |
| • |
Nucleotide mix |
| • |
Detection
reagents |
| • |
DAB Enhancer |
| • |
TACS-Nuclease |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4823-30-K |
NeuroTACS™ II Kit, 30 Samples |
TumorTACS™
is a complete reagent kit providing rapid and convenient
identification of apoptosis in tumors or cancer cells. The
labeling of tumor-containing specimens can be problematic due to
the high levels of necrosis in these tissues. During necrosis,
cessation of homeostasis, cell swelling, and nuclear membrane
rupture generates highly degraded DNA that can interfere with
interpretation of data. Trevigen’s kit has a unique TUNEL-based
system that preferentially labels the double-stranded DNA found
in apoptotic cells. DNA fragments generated during apoptosis are
end-labeled with modified nucleotides using a highly purified
terminal deoxynucleotidyl transferase enzyme (TdT) in a unique
non-toxic labeling buffer supplemented with cations for
apoptosis-specific labeling. The
 incorporated
nucleotides are subsequently detected using a horseradish
peroxidase conjugate. The conjugate catalyzes the conversion of
diaminobenzidine (DAB) into a visual dark brown precipitate. The
kit includes methyl green counterstain which is a general
counterstain appropriate for a wide variety of tissues and
tumors types and provides good contrast with the brown DAB in
labeled cells. TumorTACS contains all the reagents required for
permeabilizing, labeling, staining and counterstaining tumor
samples, including TACS-Nuclease™
for preparing positive controls. |
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Performance
tested on tumor samples. |
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample-dependent
positive controls. |
| • |
Includes DAB
Enhancer for intensifying and darkening DAB staining. |
|
| |
| • |
In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded cells and tissues. |
| • |
Assists in the
identification of apoptotic morphologies. |
| • |
Helps resolve
unique problems encountered when using tissues or cells
from tumors. |
|
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
Counterstain |
| • |
Nucleotide mix |
| • |
Detection
reagents |
| • |
DAB Enhancer |
| • |
TACS-Nuclease |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4815-30-K |
TumorTACS™ Kit, 30 Samples |
| The TiterTACS™
Colorimetric Apoptosis Detection Kit takes advantage of
Trevigen’s exclusive in situ labeling technology
bringing it to the 96 well microplate format for high throughput
quantitative detection of apoptosis. Detection using
TACS-Sapphire™, a non-toxic
colorimetric substrate, allows both kinetic and endpoint
readings. The reaction generates a blue product that can be
measured at 630 nm, and will turn yellow after the reaction is
stopped with acid allowing endpoint reading at 450 nm. During
the process of apoptosis, DNA fragmentation occurs following the
activation of endonucleases. The labeling of the 3’ ends of DNA
fragments provides an easy measure of cells undergoing
apoptosis. Modified nucleotides are incorporated at the 3’ ends
by the activity of terminal deoxynucleotidyl transferase (TdT).
These nucleotides are detected using a horseradish-peroxidase
detection system and TACS-Sapphire™.
TiterTACS™ can be used with
suspension or monolayer cells. The kit is designed to use fixed
samples, allowing you to work safely with samples that are
infected with biohazardous agents. Fixed samples may be stored
conveniently during time-course experiments.
The TiterTACS™ Kit also
provides TACS-Nuclease™
solution to generate positive controls from your own samples
giving you a maximal value for the assay. |
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| • |
Fast. Requires
less than 4 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample-dependent
positive controls. |
| • |
Convenient, 96
well microplate format. |
|
| |
| Identification and
quantitation of apoptosis in cultured cells. |
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
TACS-Nuclease
|
| • |
Nucleotide mix
|
| • |
Detection
reagents |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. Centrifuge equipped with microplate adapters. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4822-96-K |
TiterTACS™ Colorimetric, 96 Samples |
| FlowTACS™
takes advantage of Trevigen’s exclusive in situ
labeling technology to label cell samples for processing by flow
cytometry. FlowTACS™ also
provides flexibility in selection of fluorophores that are
compatible with your research design. The kit allows multi-color
labeling in conjunction with experiment specific antibodies.
DNA fragmentation is a committed step in apoptosis, and the
labeling of 3’ ends provides an easy measure of cells undergoing
apoptosis. Cells may also be analyzed for DNA content using the
included propidium iodide/RNase A solution. The FlowTACS™
Kit also provides TACS-Nuclease™
to generate positive controls for calibrating your flow
cytometer. The FlowTACS™ Kit
uses fixed cells, allowing you to safely work with cells that
are infected with biohazardous agents. Also, samples may be
stored conveniently during time-course experiments.
This complete kit provides all the reagents required for
labeling including two permeabilization reagents, labeling and
stop buffers, labeling and detection reagents, and TACS-Nuclease
for generating positive controls with your own samples. Please
refer to our components listing for details.
|
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Works on fixed
cells. |
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
TACS-Nuclease solution for preparing sample-dependent
positive controls. |
|
| |
| • |
Identify and
quantitate apoptotic cells in culture. |
| • |
Bi-color and
tri-color analysis. |
|
| |
| • |
Permeabilization reagents |
| • |
Optimized
cation |
| • |
TACS-Nuclease
|
| • |
Labeling
buffer |
| • |
Detection
reagents |
| • |
Propidium
iodide/RNase solution |
| • |
Nucleotide mix
|
| • |
Fluorescent
label |
| • |
Labeling
enzyme |
| • |
Counterstain |
|
| |
Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables.
Centrifuge equipped with microplate adapters. |
| |
| Components are stored at -20°C,
4°C, and room temperature. |
|
Products
|
4817-60-K |
FlowTACS™ Kit, 60 Samples |
The VasoTACS™
Kit was developed to provide an effective method for identifying
apoptotic cells in vascular samples. This kit allows the user to
successfully label apoptotic cells, including endothelial and
smooth muscle cells, throughout the vascular system. Similar to
the CardioTACS™ Kit, this kit
is also based on DNA end-labeling using terminal
deoxynucleotidyl transferase (TdT) and a modified nucleotide
that is subsequently detected using our TACS Blue Label™
detection system. Trevigen’s exclusive apoptosis grade TdT
enzyme coupled with the TACS Blue Label solution provides a
highly sensitive labeling method. Specifically, VasoTACS has
been tested and optimized in order to help eliminate background
and improve labeling in vascular tissue. Included in this kit is
Red Counterstain C, an excellent contrast to the TACS Blue
Label, as well as TACS-Nuclease™
that is used to generate a positive control in your own sample.
The positive control provides an internal control for
permeabilization and labeling so optimization is nominal and the
data can be interpreted with confidence. This complete kit
provides all the reagents required for labeling including two
permeabilization reagents, labeling and stop buffers, labeling
and detection reagents, TACS-Nuclease reagent, and a protocol
detailing incubation times and reagent concentrations.
|
| |
| • |
Fast. Requires
less than 3 hours to complete. |
| • |
Exclusive,
non-toxic TACS Safe TdT™
buffer - sodium cacodylate free. |
| • |
Unique buffer
system produces more consistent labeling. |
| • |
Performance
tested on vascular tissues.
|
| • |
Includes
exclusive Cytonin™
permeabilization reagent. |
| • |
Includes
exclusive Cytonin permeabilization reagent. |
|
| |
| • |
In situ
detection of apoptosis in fixed frozen, paraffin
embedded, or plastic embedded vascular cells and tissues |
| • |
Assists in the
identification of apoptotic morphologies |
| • |
Helps resolve
unique problems encountered when detecting apoptotic
vascular cells. |
|
| |
| • |
Permeabilization reagents |
| • |
Labeling
enzyme |
| • |
Chromogenic
substrate |
| • |
Labeling
buffer |
| • |
Optimized
cation |
| • |
Counterstain |
| • |
Nucleotide mix
|
| • |
Detection
reagents |
| • |
TACS-Nuclease |
|
| |
| Reagents for dehydration and
rehydration, H2O2, PBS buffer, and
disposables. |
| |
| Store components at -20°C, 4°C, and
room temperature. |
|
Products
|
4826-30-K |
VasoTACS™
Kit, 30 Reactions |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45