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| |
Poly (ADP-ribose) Polymerase (PARP)
Anti-PAR Polymer Monoclonal Antibody Now more sensitive!
Anti-PAR Polymer Polyclonal
Antibody
Anti-PARP Monoclonal Antibody
Universal Colorimetric PARP
Assay Kit
Universal
Chemiluminescent PARP Assay Kit
PARP Activity Assay Kit
Biotinylated NAD
FITC-NAD
PARP Proteins
PARP Inhibitors
| Poly (ADP-ribose)
polymerase (PARP) converts NAD into nicotinamide and polymers of
ADP-ribose at glutamic acid residues of nuclear proteins. The majority
of polymer chains are linked to PARP itself through automodification.
Poly(ADP-ribosyl)ation of subsets of nuclear proteins plays an important
role in cell DNA repair, proliferation, replication, recombination, and
apoptosis. |
| |
| The antibody was raised against purified
PAR polymer chains of 2 to 50 units long. |
| |
| The antibody is specific for poly
(ADP-ribose) polymers and does not recognize structurally related RNA,
DNA, ADP-ribose monomers, NAD or other nucleic acid monomers. |
| |
| IgG3a |
| |
| The antibody is purified from ascites fluid
and stored in unpreserved PBS buffer. The antibody’s range of use is at
dilutions of 1:500 to 1:2,000. |
| |
| • |
Western blotting
 |
| • |
ELISA
|
| • |
Immunohistochemistry
|
| • |
Immunocytochemistry |
|
| |
| Freeze in working aliquots at -20 C or -80
C in a manual defrost freezer to avoid repeated freeze-thawing. |
| |
| Bacterially expressed human PARP-treated
protein (Cat# 4500-50-P) included. |
Products
|
4335-MC-100 |
Anti-PAR
Polymer Monoclonal Antibody, 100 µl |
|
4500-50-P |
PARP
Treated Protein Control for Western Blot, 50 µl |
Poly
(ADP-ribose) polymerase (PARP) converts NAD into nicotinamide and
polymers of ADP-ribose at glutamic acid residues of nuclear proteins.
The majority of polymer chains are linked to PARP itself through
automodification. Poly(ADP-ribosyl)ation of subsets of nuclear proteins
plays an important role in cell DNA repair, proliferation, replication,
recombination, and apoptosis.
|
| |
| The antibody was raised against purified
PAR polymer chains of 10 to 50 units long. |
| |
| The antibody recognizes free poly
(ADP-ribose) and poly-ribosylated proteins. |
| |
| The antibody is prepared in rabbit and
supplied as purified IgG fraction in 1X PBS. A starting antibody
dilution of 1:1000 is recommended. |
| |
| • Western blotting |
| |
| Freeze at -20°C or -80°C in a manual
defrost freezer to avoid repeated freeze-thawing. |
Products
|
4336-BPC-100 |
Anti-PAR
Polymer Polyclonal Antibody, 100 µl |
| The anti-poly
(ADP-ribose) polymerase (PARP) mouse monoclonal antibody may be used for
the analysis of PARP protein levels and the cleavage of PARP in
apoptotic cells. |
| |
| The antibody was raised against full length
calf thymus poly (ADP-ribose) polymerase. |
| |
| It recognizes both the full length protein
(116 kDa) as well as an 85 kDa cleavage fragment associated with
apoptosis. The recognized epitope is within the C-terminal part of the
DNA binding domain of PARP. The antibody cross-reacts with human,
monkey, hamster, rat and mouse PARP, but does not recognize chicken
PARP. |
| |
| IgG1 |
| |
| The antibody is purified from mouse ascites
and is provided in PBS containing 0.2% BSA and 0.05% sodium azide. |
| |
• Western blotting
• Immunohistochemistry |
| |
| Freeze in working aliquots at -20°C in a
manual defrost freezer to avoid repeated freeze-thawing. |
| |
|
HSA PARP Enzyme
available separately. |
Products
|
4338-MC-50 |
Anti-PARP
Monoclonal Antibody (clone C2-10), 50 µg |
| Poly
ADP-ribosylation of nuclear proteins is a post-translational event that
occurs in response to DNA damage. Poly (ADP-ribose) polymerase (PARP)
catalyzes the NAD-dependent addition of poly (ADP-ribose) to adjacent
nuclear proteins. PARP plays an important role in DNA repair but can
also lead to apoptosis by depleting the cellular NAD pool. Experimental
models have shown that PARP inhibition prevents tissue damage in animal
models of myocardial and neuronal ischemia, diabetes, septic shock, and
vascular stroke. Recent data implicate a synergistic function of Ku80
and PARP-1 in minimizing chromosome aberrations and cancer development.
Trevigen's Universal 96-well PARP Assay Kit measures the incorporation
of biotinylated Poly (ADP-ribose) onto histone proteins in a 96-well
plate format. This assay is ideal for the screening of PARP inhibitors
and for measuring the activity of PARP in cell and tissue extracts. |
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| • |
Colorimetric,
non-radioactive format
|
| • |
Higher throughput 96
test size
|
| • |
Sensitivity down to
0.01 units of PARP per well |
|
| |
| • |
Identify inhibitors
and activators of PARP activity
|
| • |
Measure caspase
inactivation of PARP
|
| • |
Quantitate levels of
DNA damage in cells caused by a variety of genotoxic agents
|
| • |
Measuring activity of
PARP in cell and tissue extracts |
|
| |
| Catalog # |
Component |
Size |
4668-050-01
|
HSA PARP
Enzyme |
50 Units
|
|
4671-096-02 |
*20X PARP
Buffer |
500 µl
|
| 4671-096-03
|
*10X PARP
Cocktail** |
300 µl
|
| 4667-50-07
|
*Histone
Solution (A) |
700 µl
|
| 4667-50-03
|
*3-Aminobenzamide
|
60 µl
|
| 4672-096-P
|
Histone-Coated Plate (B) |
1 plate |
| 4671-096-04
|
10X
Strep-Diluent
|
2 µl
|
| 4800-30-06
|
Strep-HRP
|
30 µl
|
| 4822-96-08
|
TACS-Sapphire™ |
10 µl
|
| |
| Components are stored at -80°C and 4°C. |
| |
Products
|
4671-096-K |
Universal
PARP Assay Kit w/Histone Reagent, 96 samples |
|
4672-096-K |
Universal
PARP Assay Kit w/Histone Coated Plate, 96 samples |
| Poly
ADP-ribosylation of nuclear proteins is a post-translational event that
occurs in response to DNA damage. Poly (ADP-ribose) polymerase (PARP)
catalyzes the NAD-dependent addition of poly (ADP-ribose) to adjacent
nuclear proteins. PARP plays an important role in DNA repair but can
also lead to apoptosis by depleting the cellular NAD pool. Experimental
models have shown that PARP inhibition prevents tissue damage in animal
models of myocardial and neuronal ischemia, diabetes, septic shock, and
vascular stroke. Recent data implicate a synergistic function of Ku80
and PARP-1 in minimizing chromosome aberrations and cancer development.
Trevigen's Universal 96-well PARP Assay Kit measures the incorporation
of biotinylated Poly (ADP-ribose) onto histone proteins in a 96-well
plate format. This assay is ideal for the screening of PARP inhibitors
and for measuring the activity of PARP in cell and tissue extracts. |
| |
| • |
Chemiluminescent,
non-radioactive format
|
| • |
Higher throughput 96
test size
|
| • |
sensitivity down to
0.0025 units of PARP per well |
|
| |
| • |
Identify inhibitors
and activators of PARP activity
|
| • |
Measure caspase
inactivation of PARP
|
| • |
Quantitate levels of
DNA damage in cells caused by a variety of genotoxic agents
|
| • |
Measuring activity of
PARP in cell and tissue extracts |
|
| |
| Catalog # |
Component |
Size |
4668-50-01
|
PARP-HSA |
50 µl
|
|
4671-096-02 |
*20X PARP
Buffer |
500 µl
|
| 4671-096-03
|
*10X PARP
Cocktail** |
300 µl
|
|
4671-096-05 |
5X Coating
Buffer (A) |
1.4 ml
|
| 4667-50-07
|
*Histone
Solution (A) |
700 µl
|
| 4667-096-P
|
*Histone-Coated Strip wells (B) |
96 |
| 4667-50-03
|
*3-Aminobenzamide |
60 µl
|
|
4671-096-04 |
10X
Strep-Diluent |
2 ml
|
| 4800-30-06
|
Strep-HRP |
30 µl
|
|
4675-096-01 |
PeroxyGlow™
A |
6 ml
|
| 4800-30-06
|
PeroxyGlow™
B |
6 ml
|
|
4671-096-06 |
*10X
Activated DNA |
300 µl
|
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| Components are stored at -80°C and 4°C. |
| |
Products
|
4675-096-K |
Universal
Chemiluminescent PARP Assay Kit with Histone Reagent (96 samples) |
|
4676-096-K |
Universal
Chemiluminescent PARP Assay Kit with Histone Coated Plate (96 samples) |
|
4667-50-K |
PARP
Assay Kit, 50 Samples |
| Trevigen’s
biotinylated NAD (6-biotin-17-nicotinamide-adenine-dinucleotide)
provides a convenient non-isotopic alternative to radiolabeled NAD for
studies requiring this substrate. A number of proteins, including poly
(ADP-ribose) polymerase (PARP), use NAD as a substrate for their
function. The PARP enzyme catalyzes the polymerization of ADP-ribose
onto a number of nuclear proteins. Biotinylated NAD allows an indirect
measure of PARP activity when biotin incorporation is detected using a
conjugated-streptavidin detection system. |
| |
| • |
Activity measurements
for NAD requiring enzymes
|
| • |
Assays to identify
PARP inhibitors |
|
| |
| Provided as a buffered solution
containing 250 µM 6-biotin-17-nicotinamide-adenine-dinucleotide, sodium
salt. |
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| 1179 g/mol |
| |
22,000 at 265 nm
|
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| >95% by HPLC |
| |
| Store at -80°C in working
aliquots to avoid repeated freeze-thawing. |
Products
|
4670-500-01 |
Biotinylated NAD, 500 µl |
|
4670-500-05 |
Biotinylated NAD, 5 x 500 µl |
|
4670-500-10 |
Biotinylated NAD, 10 x 500 µl |
 F
NAD for use with enzymes requiring NAD as a substrate or cofactor. A
number of proteins, including poly (ADP-ribose) polymerase (PARP), and
the SIR2 family of NAD(+)-dependent histone/protein deacetylases, use
NAD as a substrate for their function. FITC conjugated NAD permits the
direct measurement of PARP and other NAD-dependent enzymes by
fluorescence microscopy or by incorporation of fluorescent-labeled poly
(ADP-ribose) or O-acety-ADP ribose onto histones attached to a 96 well
plate. |
| |
| • |
Activity measurements
for NAD requiring enzymes |
| • |
Assays to identify
inhibitors of NAD-requiring enzymes |
| • |
Cell Permeabilization
reagent, 4674-250-01, required for in-cell assays |
|
| |
| Provided in solution at a
concentration of 250 µm in deionized water. There is a 1:1 stoichiometry
for incorporation; one FITC label for each NAD molecule. |
| |
| 21,000 at 265nm |
| |
95% by HPLC
|
| |
| Store at -80°C in working
aliquots to avoid repeated freeze-thawing. |
Products
|
4673-500-01 |
FITC-NAD,
250 µl |
|
4673-500-05 |
FITC-NAD,
5 x 250 µl |
|
4673-500-10 |
FITC-NAD,
10 x 250 µl |
| Trevigen now
offers two grades of poly (ADP-ribose) polymerase: a “standard” PARP,
and a new high specific activity (HSA) enzyme. The specific activity
serves as a measure of enzyme purity, with the standard enzyme being
purified to approximately 50% purity, and the high specific activity
enzyme to greater than 95%. The standard PARP enzyme is useful as a
positive control for both Western blot analysis of ribosylated proteins
and the PARP Activity Assay (Cat# 4667-50-K). For more detailed studies
or for drug discovery applications, the HSA PARP is recommended. The
catalytic domain of PARP is also available. |
| |
| One unit is the amount of
enzyme required to incorporate 100 pmol of poly(ADP) from NAD into
acid-insoluble form in 1 minute at 22°C. |
| |
| Purified from E. coli
containing a recombinant plasmid encoding the full-length human PARP
gene. |
| |
| • |
Identification of
inhibitors and activators of PARP activity
|
| • |
Quantitation of DNA
damage
|
| • |
Investigation of PARP
inactivation during apoptosis |
|
| |
| Freeze in working aliquots at
-80 C to avoid repeated freeze-thawing. |
Products
|
4667-50-01 |
PARP
Enzyme, 100 Units |
|
4667-250-01 |
PARP
Enzyme, 500 Units |
|
4668-100-01 |
PARP
Enzyme, High Specific Activity, 100 Units |
|
4668-500-01 |
PARP
Enzyme, High Specific Activity, 500 Units |
|
4668-2K-01 |
PARP
Enzyme, High Specific Activity, 2000 units |
|
4669-100-01 |
Chicken
PARP Catalytic Domain, 100µg |
|
* 4669-100-02 |
Human
PARP Catalytic Domain, 100µg |
 3-Aminobenzamide |
3-aminobenzamide is the
PARP inhibitor provided with Trevigen's PARP Inhibition Assay Kit (Cat#
4669-96-K) at 200 mM.
|
|
4-Amino-1,8-naphthalimide |
4-Amino-1,8-naphthalimide is a potent inhibitor of poly(ADP-ribose)
polymer (PARP) and reduces ischemia-reperfusion injury in the heart and
skeletal muscle. It is 1000-fold more potent that 3-aminobenzamide and
exhibits mixed-type inhibition with respect to the sub-strate, NAD+, at
micromolar concentrations. This inhibitor, which has been analyzed at a
concentration of 20 µM, is provided at a convenient concentration for
subsequent serial dilution and use with Trevigen's PARP Inhibition Assay
Kit (Cat# 4669-96-K).
|
|
6(5H)-Phenanthridinone |
6(5H)-Phenanthridinone
strongly inhibits poly(ADP-ribose) polymerase (PARP) and displays
immunosuppressive activity. It is a mixed-type inhibitor that acts on
both the enzyme and enzyme-NAD+ complex at site(s) distinct from the
NAD+-binding site to attenuate the fall in NAD and ATP and,
consequently, improve cell survival. It also inhibits concanavalin
A-induced lymphocyte proliferation at micromolar concentrations. This
inhibitor, which has been analyzed in the range of 0.18-0.39 µM, is
provided at a convenient concentration for subsequent serial dilution
and use with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).
|
|
Benzamide |
| Benzamide is the most
potent poly(ADP-ribose) polymerase (PARP) inhibitor in the family of
benzamides. It acts as a neuroprotectant since it inhibits PARP, an
enzyme activated by nitric oxide. Benzamide is twice as active than its
commonly used counterpart, 3-aminobenzamide in delaying or suppressing
PARP activation. It is able to prevent nuclear fragmentation and
apoptotic-body formation without affecting DNA fragmentation during
apoptosis. This PARP inhibitor, which has been analyzed in the range of
100 to 500 µM, is provided at a convenient concentration for subsequent
serial dilution and use with Trevigen's PARP Inhibition Assay Kit (Cat#
4669-96-K). |
Products
|
4667-50-03 |
3-Aminobenzamide, 60 µl |
|
4667-50-09 |
4-Amino-1,8-naphthalimide 100 µl |
|
4667-50-10 |
6(5H)-Phenanthridinone, 100 µl |
|
4667-50-11 |
Benzamide, 100 µl |
|
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