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New Oxidative Damage Products
Anti-8-oxo-dG Monoclonal Antibody (Clone 1F7)
Anti-8-oxo-dG Monoclonal Antibody (Clone 4E9)
Anti-8-oxo-dG Monoclonal Antibody (Clone 4A6)
Anti-8-oxo-dG Monoclonal Antibody (Fab 166)
Superoxide Dismutase
Assay Kit
Glutathione Reductase
Assay Kit
Glutathione Assay Kit
Glutathione Peroxidase
Assay Kit
| The anti-8-oxo-dG antibody allows
detection and quantitation of 7,8-dihydro-8-oxo-guanine (8-oxo-dG,
7,8-dihydro-8-oxo-guanine, or 7-oxo-7,8-dihydroguanine) in DNA, cell,
and tissue samples. The 8-oxo-dG lesion is the most common form of
base change caused by oxidative stress. Attack by free radical oxygen
occurs at the N7-C8 bond of guanine resulting in the formation of the
oxidized base.
DNA polymerases preferentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore, without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesions result in mutational frequencies of
1-5% (mainly G:C to T:A transitions).
|
| 7,
8-dihydro-8-oxo-guanine. |
| |
|
| The
antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. Cross
reaction with guanine and guanosine occurs at concentrations 800
to 20,000-fold higher than for 7,8-dihydro-8-oxo-guanine. The
antibody will cross react with structurally related
7,8-dihydro-8-oxo-guanine derivatives, e.g. 5-
hydroxymethyluridine. Limited cross reaction with denatured calf
thymus has been observed, and as a result, this should be
avoided as a blocking agent in immunocytochemistry. |
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| IgG1 |
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| The
antibody is purified from mouse ascites and is provided in
phosphate buffered saline (PBS) containing 0.01% sodium azide. |
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| • |
Immunocytochemistry
 |
| • |
ELISA |
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| Store at 4°C.
For long term storage, divide into working aliquots and freeze
at -20°C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
|
4355-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody clone 1F7,100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzeihmer's
disease, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo-dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond by oxidative
radicals. DNA polymerases preferentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore, without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1- 5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| Purified IgG is
provided in phosphate buffered saline with 0.01% sodium azide as
preservative. |
| |
|
| 7,
8-dihydro-8-oxo-guanine containing dinucleotide coupled to BSA. |
| |
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| The antibody has
similar binding affinity for 8- hydroxydeoxyguanosine and for
7,8-dihydro-8-oxo guanine. This antibody recognizes the 8-oxoG
as a single nucleotide. |
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|
| IgG |
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| • |
Immunostaining
|
| • |
ELISA |
|
| Store at 4°C for
short term. For long term storage, divide into working aliquots
and freeze at -20°C in a manual defrost freezer to avoid
repeated freeze-thawings. |
|
Products
|
4359-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody (Clone 4E9), 100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzheimer's
diseaseą, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo0dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond of guanine by
oxidative radicals. DNA polymerases prefentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1-5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| 7,
8-dihydro-8-oxo-guanine containing dinucleotide coupled to BSA |
| |
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|
This antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. This
antibody recognizes the 8-oxo-G in single or double standard DNA
settings. |
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| IgG1 |
|
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|
| The
antibody is purified from mouse ascites and is provided in
phosphate buffered saline (PBS) containing 0.01% sodium azide as
a preservative. |
| |
|
| • |
Immunocytochemistry
|
| • |
ELISA |
|
| Store at 4°C.
For long term storage, divide into working aliquots and freeze
at -20°C in a manual defrost freezer to avoid repeated
freeze-thawings. Do not freeze and thaw aliquots more than once.
|
|
Products
|
4357-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody clone 4A6, 100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzheimer's
diseaseą, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo0dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond of guanine by
oxidative radicals. DNA polymerases prefentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1-5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| Store at 4°C for
short term. Aliquot and freeze at -20°C for long term storage.
Do not freeze and thaw aliquots more than once. |
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| lgG, Fab fragment |
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| Purified Fab fragment
provided in PBS with 0.01% sodium azide as preservative. |
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| The
antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. Cross
reaction with guanine and guanosine shown in Table 1 |
|
Products
|
4358-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody FAB 166, 100 µg |
Superoxide Dismutase (SOD) catalyzes
the dismutation of the superoxide radical (O2-)
into hydrogen peroxide (H202) and elemental oxygen
(O2), and as such, provides an important defense against the
toxicity of superoxide radical. Overexpression of SOD protects murine
fibrosarcoma cells from apoptosis and promotes cell differentiation (1).
SOD also inhibits adriamycin-induced apoptosis in murine peritoneal
macrophages (2). Superoxide ions, generated from the conversion of
xanthine to uric acid and hydrogen peroxide by xanthine oxidase,
converts NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm.
SOD reduces the superoxide ion concentration and thereby lowers the rate
of NBT-diformazan formation. The extent of reduction in the appearance
of NBT-diformazan is a measure of SOD activity present in your
experimental sample. The assay is free of interference by other
catalytic activities, and is ideal for assaying SOD in mammalian cell
lysates. The kit contains the proper lysis buffer and the reagents
needed for 100 experimental tests, 50 positive controls, and 50 negative
controls. Unlike some other assay kits for SOD, this system is not
greatly disturbed by trace metals. Each assay requires only about five
minutes, and after a simple calculation, the percent inhibition of the
formation of NBT-diformazan by SOD is converted to the relative activity
of the sample.
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| • |
Suitable for
mammalian cells
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| • |
Each sample
takes only 5 minutes
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| • |
Contains SOD
for 50 positive controls
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| • |
Suitable for
the assay of (Mn2+)-SOD, (Fe2+)-SOD,
and (Cu/Zn)-SOD |
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| Calculating SOD
activity by measuring the reduction of NBT-diformazan in cell
extracts. |
| Catalog # |
Component |
Size |
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7500-100-01
|
SOD
(1 unit/µl) |
200
µl |
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7500-100-02 |
25X SOD Reaction Buffer |
12
ml |
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7500-100-03 |
Xanthine Solution |
1.5
ml |
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7500-100-04 |
NBT Solution
|
6
ml |
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7500-100-05 |
XOD
Solution |
2
ml |
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7500-100-06 |
20X Cell Lysis Solution |
12
ml |
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| Store components at 4°C. |
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Products
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7500-100-K |
Superoxide Dismutase Assay Kit, 100 Reactions |
Trevigen is pleased to announce the
release of a new, higher volume assay kit designed to measure cellular
levels of Glutathione Reductase, an enzyme which catalyzes the reduction
of oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH is an
important intracellular antioxidant that reacts with oxygen free
radicals and organic peroxides and acts as a substrate for other
detoxification enzymes such as glutathione peroxidase and glutathione
S-transferase. The activity of glutathione reductase is an important
measure of
the antioxidant status of the cell. Glutathione reductase requires NADPH
for its activity, resulting in the reduction of GSSG to GSH and the
corresponding oxidation of NADPH to NADP.
Trevigen’s Glutathione Reductase Assay takes less than 6 minutes to
perform and is based on the measurement of the loss in absorbance at 340
nm due to the conversion of NADPH to NADP+. The kit contains everything
that you need to perform the assay on 100 samples and its corresponding
blanks, each in duplicate, as well as 10 standard curves with each point
performed in duplicate. In total, each kit can generate data on over 500
data points. The NADPH is provided lyophilized in 10 vials, each
containing sufficient NADPH for generating one standard curve and for
determining glutathione reductase levels in 10 experimental samples.
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| • |
Suitable for
cell lysates, erythrocyte lysates, and tissue homogenate
|
| • |
Each sample
takes only 6 minutes
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| • |
Sufficient
reagents for 100 assays and 10 standard curves |
|
|
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|
| Calculating
glutathione reductase activity. |
| Catalog # |
Component |
Size |
| |
7510-100-01
|
Glutathione Reductase |
500
µl |
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7510-100-02 |
25X Reaction Buffer |
20
ml |
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7510-100-03 |
NADPH |
10
x 9.2 mg |
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7510-100-04 |
GSSG Solution |
6
ml |
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7510-100-05 |
25X
Sample Dilution Buffer |
20
ml |
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7510-100-06 |
25X Tissue Homogenization Buffer |
40
ml |
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| Store components at 4°C. NADPH
should be stored at -20°C. |
|
Products
|
7510-100-K |
Glutathione Reductase Assay, 100 Assays |
| Trevigen’s Glutathione assay kit
utilizes a carefully optimized enzymatic recycling method, using
Glutathione reductase, for the quantification of Glutathione. The
sulfhydryl group of Glutathione reacts with DTNB
(5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) and produces a
yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide,
GSTNB (between GSH and TNB) that is concomitantly produced, is reduced
by Glutathione reductase to recycle the GSH and produce more TNB. The
rate of TNB production is directly proportional to this recycling
reaction which is in turn directly proportional to the concentration of
GSH in the sample. Measurement of the absorbance of TNB at 405 or 414 nm
provides an accurate estimation of Glutathione in the sample. |
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| • |
Suitable for
mammalian cells, tissue, blood, plasma and other bodily
fluids
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| • |
Formatted for
96-well plates
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| • |
Contains
sufficient reagents to assay 400 data points
or to determine GSH in:
|
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|
(a) |
120
experimental samples, each performed in
triplicate, plus one GSH standard curve
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(b) |
96
experimental samples, each performed in
triplicate, plus 4 GSH standard curves
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(c) |
64
experimental samples, each performed in
triplicate, plus 8 GSH standard curves
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| • |
Includes
procedure for determining oxidized and reduced
glutathione |
|
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| Calculating total,
reduced, and oxidized glutathione concentrations. |
| Catalog # |
Component |
Size |
| |
7511-100-01
|
Glutathione Reductase |
80
µl |
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7511-100-02 |
25X Assay Buffer |
12.0 ml |
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7511-100-03 |
Glutathione Standard |
20
nmoles |
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7511-100-04 |
Reaction Mix |
8
bottles |
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7511-100-05 |
96-well plates |
8
plates |
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| Store components at 4°C. |
|
Products
|
7511-100-K |
Glutathione Assay Kit, 100 Assays |
| Glutathione peroxidase is a tetramer
of four identical subunits, with a molecular weight of 84,000. It
requires selenium as a cofactor and contains a selenocysteine amino acid
residue in the active site of each monomer that participates in the
actual mechanism of the enzyme. Glutathione peroxidase (GP) is found in
mammalian cells and helps to prevent lipid peroxidation of cell
membranes by consuming free peroxide in the cell. Trevigen’s Glutathione
Peroxidase Assay Kit can be used to measure glutathione-dependent
peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell
lysates. |
| |
| • |
Suitable for a 96 well or
cuvette format
 |
| • |
Suitable for plasma,
erythrocyte lysates, tissue homogenates, and cell
lysates
|
|
|
| |
|
| Calculating
glutathione peroxidase activity. |
| Catalog # |
Component |
Size |
| |
7512-100-01 |
Glutathione
Peroxidase |
800 µl |
| |
7512-100-02 |
10X Assay Buffer |
20 ml |
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7512-100-03 |
GSH + NADPH |
10 vials |
| |
7512-100-04 |
Glutathione
Reductase |
1.1 ml |
| |
7512-100-05 |
Cumene
Hydroperoxide |
12 ml |
| |
7512-100-06 |
96-well plates |
5 plates |
| |
| Components are stored at -20°C and
room temperature. |
| |
1. Ozdemir G, Ozden M, Maral H,
Kuskay S, Cetinalp P, Tarkun I. Malondialdehyde, glutathione,
glutathione peroxidase and homocysteine levels in type 2
diabetic patients with and without microalbuminuria. Ann Clin
Biochem. 2005, 42:99-104.
2. Prasad NR, Menon VP, Vasudev V, Pugalendi KV. Radioprotective
effect of sesamol on gamma-radiation induced DNA damage, lipid
peroxidation and antioxidants levels in cultured human
lymphocytes. Toxicology. 2005, 209:225-35.
3. Sindhu RK, Ehdaie A, Farmand F, Dhaliwal KK, Nguyen T, Zhan
CD, Roberts CK, Vaziri ND. Expression of catalase and
glutathione peroxidase in renal insufficiency. Biochim Biophys
Acta. 2005, 1743:86-92 |
|
Products
|
7512-100-K |
Glutathione Peroxidase Assay Kit, 100 Assays |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45