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Klenow Fragment

Klenow Enzyme
 
The large fragment of E. coli DNA polymerase I (Klenow) is a DNA polymerase with an associated 3’ to 5’ exonuclease activity and lacks any 5’ to 3’ exonuclease activity. This enzyme is used in a variety of experiments including DNA end labeling of 3’ recessed ends and DNA sequencing.
 
Concentration: 15 units/µl.
 
Unit Definition: 1 unit incorporates 10 nmole of total nucleotide into acid-insoluble form in 30 minutes at 37°C using activated DNA as a template/primer.
 
Applications:
•   DNA end labeling of 3' recessed ends
•   In situ labeling of apoptotic cells
 
Source: Purified from a recombinant E. coli strain.
 
Storage: Store at -20°C in a manual defrost freezer to avoid repeated freeze-thawing.

Products

9520-500-01 Klenow Enzyme, 500 Units

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07