Instant mRNA Capture Discs

Now it is possible to isolate mRNA faster than total RNA, using Trevigen’s unique INSTANT mRNA Capture Discs. These discs are coated with a proprietary, non-oligo dT-based coating that selectively captures poly-adenylated RNA directly from cell lysates. The entire procedure can be completed in less than 1 hour. The discs are provided in individual RNase-free tubes. Simply incubate in Blocking Solution for 30 minutes, add cell lysate (prepared in the provided guanidine thiocyanate solution), incubate for 10 to 30 minutes, and finally rinse the disc in Wash Buffer. The purified mRNA is free from DNA, polysomal, and total RNA. The filter discs can be dried for storage, used directly in RT-PCR experiments, or the mRNA can be removed from the discs by heating.

Each kit is provided with Lysis Solution containing guanidine thiocyanate which has been demonstrated to inactivate ribonucleases very rapidly, assuring the highest quality RNA isolation. RNase free Disc Picks are included to quickly transfer and wash the discs, resulting in purified mRNA with minimal hands-on effort. mRNA isolated on INSTANT mRNA Capture Discs is ready to be used in RT-PCR. A disc can be quartered for analysis of only part of the sample, allowing future analysis of the same sample, or analysis with multiple sets of primers.

Since no columns are used, or precipitation required, the loss of mRNA during the procedure is minimized. This methodology allows the use of smaller amounts of starting material for analysis. In fact, there is no theoretical lower limit to the number of cells required as long as the mRNA species is present.