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| |
Fragment Length Analysis by Repair Enzymes (FLARE)
FLARE™ Assay Kits
Fpg FLARE™ Assay Kit and Module
Endonuclease III FLARE™ Assay Kit and Module
hOGG1 FLARE™ Assay Kit and Module
| Trevigen’s unique FLARE™
(Fragment Length Analysis using Repair Enzymes) Assays provide the
ability to detect DNA damage in single cells using a variety of DNA
repair enzymes in conjunction with Trevigen’s CometAssay™
single cell gel electrophoresis kit. To assess the type of DNA damage
induced by a putative mutagen, drug, or treatment regimen, cells are
harvested after treatment and immobilized in a layer of low melting
point agarose on the FLARE Slide. |
| |
| • |
FLARE Slides
allow direct application of low melting point agarose
without base layers or pretreatment for three samples.
 |
| • |
Hydrophobic
barrier allows easy application of cells directly to
slide and treatment with enzyme.
|
| • |
Base specific
DNA damage detection.
|
| • |
Convenient
format. |
|
| |
| • |
CometAssay™
|
| • |
DNA Damage
Detection |
|
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|
|
Catalog # |
FLARE Assay |
Damage Recognized |
|
|
4040-100-FK
4040-100-FM |
Fpg |
8-oxoguanine, DNA containing
formamidopyrimidine moieties |
|
 |
4045-100-FK
4045-100-FM |
Endonuclease III |
Thymine Glycol, 5,6-dihydrothymine, urea,
5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan,
5-hydroxy-6-hydrouracil, uracil glycol,
5-hydroxy-5-methylhydantoin,
5-hydroxycytosine,5-hydroxyuracil,methyltartonylurea,
thymine ring saturated or fragmentation product |
4130-100-FK
4130-100-FM |
hOGG1 |
8-oxoguanine, DNA containing
formamidopyrimidine moieties |
 |
4055-100-FK
4055-100-FM |
T4-PDG |
Cis-syn isomers of cyclobutane pyrimidine
dimers |
|
 |
4065-100-FK
4065-100-FM |
cv-PDG |
Cis-syn and trans-syn isomers of
cyclobutane pyrimidine dimers |
4100-100-FK
4100-100-FM |
UVDE |
Cyclobutane pyrimidine dimers, (6-4)
photoproducts |
|
| |
|
| Store components at -80°C, -20°C,
4°C, and room temperature. |
|
Products
|
3950-075-02 |
FLARE Slides, 25 slides |
|
3950-300-02 |
FLARE Slides, 100 slides |
Trevigen's Fpg FLARE (Fragment
Length Analysis using Repair Enzymes) Assay provides the ability to
probe for DNA damage in single cells using E. coli
Formamidopyrimidine-DNA Glycosylase (Fpg) in conjunction with Trevigen's
CometAssay™ single cell gel
electrophoresis kit. The Fpg FLARE Assay Kit supplies the enzyme,
reagents, and FLARE Slides to perform the CometAssay. The FLARE Module
only provides the enzyme and buffers for use with the CometAssay.
|
| |
| Fpg FLARE™
Assay Kit |
| Catalog # |
Component |
Size |
| |
4040-100-01
|
E. coli Fpg
|
500
Units |
| |
3950-040-01
|
25X FLARE™
Buffer 1 |
40 ml |
| |
3950-010-03 |
REC™
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
3950-075-02 |
FLARE Slides |
25
each |
| |
4250-050-01 |
Lysis Solution |
2
x 500 ml |
| |
4250-050-02 |
Comet LMAgarose (LMA) |
15
ml |
| |
4250-050-05 |
**SYBR®
Green I nucleic acid gel stain |
5
µl |
| |
|
|
|
| Fpg FLARE™
Module |
| Catalog # |
Component |
Size |
| |
4040-100-01 |
E. coli Fpg
|
500
Units |
| |
3950-040-01 |
25X FLARE™
Buffer 1 |
40
ml |
| |
3950-010-03 |
REC™
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
|
Products
|
4040-100-FK |
Fpg FLARE Assay Kit, 75 Samples |
|
4040-100-FM |
Fpg FLARE Module, 100 Samples |
| Trevigen's Endonuclease III FLARE
(Fragment Length Analysis using Repair Enzymes) Assay provides the
ability to detect DNA damage in single cells using E. coli
Endonuclease III (Thymine Glycol-DNA Glycosylase) in conjunction with
Trevigen's CometAssay™ single cell gel
electrophoresis kit. The Endonuclease III FLARE Assay Kit supplies the
enzyme, reagents, and FLARE Slides to perform the CometAssay. The FLARE
Module only provides the enzyme and buffers for use with the CometAssay.
|
| |
| Endonuclease III FLARE™
Assay Kit |
| Catalog # |
Component |
Size |
| |
4045-100-01
|
E. coli Endonuclease
III |
100
Units |
| |
3950-040-01 |
25X FLARE Buffer 1 |
40
ml |
| |
3950-010-03 |
REC
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
3950-075-02 |
FLARE Slides |
25
each |
| |
4250-050-01 |
Lysis Solution |
2
x 500 ml |
| |
4250-050-02 |
Comet LMAgarose (LMA) |
15
ml |
| |
4250-050-05 |
**SYBR®
Green I nucleic acid gel stain |
5
µl |
| |
|
|
|
| Endonuclease III FLARE™
Module |
| Catalog # |
Component |
Size |
| |
4045-100-01
|
E. coli Endonuclease
III |
100
Units |
| |
3950-040-01 |
25X FLARE Buffer 1 |
40
ml |
| |
3950-010-03 |
REC
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
|
Products
|
4045-100-FK |
Endonuclease III FLARE™ Assay Kit, 75 Samples |
|
4045-100-FM |
Endonuclease III FLARE™ Module, 100 Samples |
| Trevigen's hOGG1 FLARE (Fragment
Length Analysis using Repair Enzymes) Assay provides the ability to
probe for DNA damage in single cells using hOGG1 in conjunction with
Trevigen's CometAssay™ single cell gel
electrophoresis kit. The hOGG1 FLARE Assay Kit supplies the enzyme,
reagents, and FLARE Slides to perform the CometAssay. The FLARE Module
only provides the enzyme and buffers for use with the CometAssay. |
| |
| hOGG1 FLARE™
Assay Kit |
| Catalog # |
Component |
Size |
| |
4130-100-01
|
hOGG1 100 Units
|
25X
|
| |
3950-040-01 |
FLARE Buffer 1 |
40
ml |
| |
3950-010-03 |
REC
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
3950-075-02 |
FLARE Slides |
25
each |
| |
4250-050-01 |
Lysis Solution |
2
x 500 ml |
| |
4250-050-02 |
Comet LMAgarose (LMA) |
15
ml |
| |
4250-050-05 |
**SYBR®
Green I nucleic acid gel stain |
5
µl |
| |
|
|
|
| hOGG1 FLARE™
Assay Module |
| Catalog # |
Component |
Size |
| |
4130-100-01
|
hOGG1 |
100
Units |
| |
3950-040-01 |
25X FLARE Buffer 1 |
40
ml |
| |
3950-010-03 |
REC
Dilution Buffer |
10
ml |
| |
3950-100-04 |
100X BSA Additive |
100 µl |
| |
|
Products
|
4130-100-FK |
hOGG1 FLARE™ Assay Kit, 75 Samples |
|
4130-100-FM |
hOGG1 FLARE™ Module, 100 Samples |
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