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Fragment Length Analysis by Repair Enzymes (FLARE™)

FLARE™ Assay Kits
Fpg FLARE™ Assay Kit and Module
Endonuclease III FLARE™ Assay Kit and Module
hOGG1 FLARE™ Assay Kit and Module
T4-PDG FLARE™ Assay Kit and Module
cv-PDG FLARE™ Assay Kit and Module
UVDE FLARE™ Assay Kit and Module

FLARE™ Assay Kits

FLARE™ Assay Kits
 
Trevigen’s unique FLARE™ (Fragment Length Analysis using Repair Enzymes) Assays provide the ability to detect DNA damage in single cells using a variety of DNA repair enzymes in conjunction with Trevigen’s CometAssay™ single cell gel electrophoresis kit. To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in a layer of low melting point agarose on the FLARE Slide.
 
Features:
•   FLARE Slides allow direct application of low melting point agarose without base layers or pretreatment for three samples.
•   Hydrophobic barrier allows easy application of cells directly to slide and treatment with enzyme.
•   Base specific DNA damage detection.
•   Convenient format.
 
Application:
•   CometAssay™
•   DNA Damage Detection
     
Components:
Catalog # FLARE Assay Damage Recognized    
4040-100-FK
4040-100-FM		 Fpg 8-oxoguanine, DNA containing formamidopyrimidine moieties  
4045-100-FK
4045-100-FM		 Endonuclease III Thymine Glycol, 5,6-dihydrothymine, urea, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, alloxan, 5-hydroxy-6-hydrouracil, uracil glycol, 5-hydroxy-5-methylhydantoin, 5-hydroxycytosine,5-hydroxyuracil,methyltartonylurea, thymine ring saturated or fragmentation product
4130-100-FK
4130-100-FM		 hOGG1 8-oxoguanine, DNA containing formamidopyrimidine moieties
4055-100-FK
4055-100-FM		 T4-PDG Cis-syn isomers of cyclobutane pyrimidine dimers  
4065-100-FK
4065-100-FM		 cv-PDG Cis-syn and trans-syn isomers of cyclobutane pyrimidine dimers
4100-100-FK
4100-100-FM		 UVDE Cyclobutane pyrimidine dimers, (6-4) photoproducts
     
Storage: Store components at -80°C, -20°C, 4°C, and room temperature.

Products

3950-075-02 FLARE Slides, 25 slides
3950-300-02 FLARE Slides, 100 slides


Fpg FLARE™ Assay Kit and Module

Fpg FLARE™ Assay Kit and Modules
 
Trevigen's Fpg FLARE (Fragment Length Analysis using Repair Enzymes) Assay provides the ability to probe for DNA damage in single cells using E. coli Formamidopyrimidine-DNA Glycosylase (Fpg) in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The Fpg FLARE Assay Kit supplies the enzyme, reagents, and FLARE Slides to perform the CometAssay. The FLARE Module only provides the enzyme and buffers for use with the CometAssay.
 
 
Components: Fpg FLARE™ Assay Kit
  Catalog # Component Size
  4040-100-01
 		 E. coli Fpg 500 Units
  3950-040-01 25X FLARE™ Buffer 1 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-075-02 FLARE Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
       
Components: Fpg FLARE™ Module
  Catalog # Component Size
  4040-100-01 E. coli Fpg
 		 500 Units
  3950-040-01 25X FLARE™ Buffer 1 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
   
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4040-100-FK Fpg FLARE Assay Kit, 75 Samples
4040-100-FM Fpg FLARE Module, 100 Samples


Endonuclease III FLARE™ Assay Kit and Module

Endonuclease III FLARE™ Kit and Module
 
Trevigen's Endonuclease III FLARE (Fragment Length Analysis using Repair Enzymes) Assay provides the ability to detect DNA damage in single cells using E. coli Endonuclease III (Thymine Glycol-DNA Glycosylase) in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The Endonuclease III FLARE Assay Kit supplies the enzyme, reagents, and FLARE Slides to perform the CometAssay. The FLARE Module only provides the enzyme and buffers for use with the CometAssay.
 
Components: Endonuclease III FLARE™ Assay Kit
  Catalog # Component Size
  4045-100-01
 		 E. coli Endonuclease III 100 Units
  3950-040-01 25X FLARE Buffer 1 40 ml
  3950-010-03 REC Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-075-02 FLARE Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
       
Components: Endonuclease III FLARE™ Module
  Catalog # Component Size
  4045-100-01
 		 E. coli Endonuclease III 100 Units
  3950-040-01 25X FLARE Buffer 1 40 ml
  3950-010-03 REC Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
   
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4045-100-FK Endonuclease III FLARE™ Assay Kit, 75 Samples
4045-100-FM Endonuclease III FLARE™ Module, 100 Samples


hOGG1 FLARE™ Assay Kit and Module

hOGG1 FLARE™ Assay Kit and Module
 
Trevigen's hOGG1 FLARE (Fragment Length Analysis using Repair Enzymes) Assay provides the ability to probe for DNA damage in single cells using hOGG1 in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The hOGG1 FLARE Assay Kit supplies the enzyme, reagents, and FLARE Slides to perform the CometAssay. The FLARE Module only provides the enzyme and buffers for use with the CometAssay.
 
Components: hOGG1 FLARE™ Assay Kit
  Catalog # Component Size
  4130-100-01
 		 hOGG1 100 Units
 		 25X
  3950-040-01 FLARE Buffer 1 40 ml
  3950-010-03 REC Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-075-02 FLARE Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
       
Components: hOGG1 FLARE™ Assay Module
  Catalog # Component Size
  4130-100-01
 		 hOGG1 100 Units
  3950-040-01 25X FLARE Buffer 1 40 ml
  3950-010-03 REC Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
   
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4130-100-FK hOGG1 FLARE™ Assay Kit, 75 Samples
4130-100-FM hOGG1 FLARE™ Module, 100 Samples

 

T4-PDG FLARE™ Assay Kit and Module

T4-PDG FLARE™ Assay Kit and Module
 
Trevigen's T4-PDG FLARE™ (Fragment Length Analysis using Repair Enzymes) Assay Kit provides the ability to probe for DNA damage in single cells using T4 phage pyrimidine dimer glycosylase (T4-PDG) in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The T4-PDG FLARE Assay Kit supplies the enzyme, reagents, and FLARE Slides to perform the CometAssay™. The FLARE Module only provides the enzyme and buffers for use with the CometAssay™.
 
Components: T4-PDG FLARE™ Assay Kit
  Catalog # Component Size
  4055-100-01
 		 T4-PDG 100 Units
  3952-040-01 25X FLARE™ Buffer 3 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-075-02 FLARE™ Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
   
Components: T4-PDG FLARE™ Module
  Catalog # Component Size
  4055-100-01
 		 T4-PDG 100 Units
  3952-040-01 25X FLARE™ Buffer 3 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
       
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4055-100-FK T4-PDG FLARE™ Assay Kit, 75 Samples
4055-100-FM T4-PDG FLARE™ Module, 100 Samples


cv-PDG FLARE™ Assay Kit and Module

cv-PDG FLARE™ Assay Kit and Module
 
Trevigen's cv-PDG FLARE™ (Fragment Length Analysis using Repair Enzymes) Assay Kit provides the ability to probe for DNA damage in single cells using chorella virus pyrimidine dimer glycosylase (cv-PDG) in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The cv-PDG FLARE Assay Kit provides necessary reagents and FLARE Slides to perform the CometAssay along with enzyme and dilution buffers. The FLARE Module provides the enzyme along with the dilution buffer for use with the CometAssay.
 
Components: cv-PDG FLARE™ Assay Kit
  Catalog # Component Size
  4065-100-01
 		 cv-PDG 100 Units
  3952-040-01 25X FLARE™ Buffer 3 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-075-02 FLARE™ Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
   
Components: cv-PDG FLARE™ Assay Module
  Catalog # Component Size
  4065-100-01
 		 cv-PDG 100 Units
  3952-040-01 25X FLARE™ Buffer 3 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
       
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4065-100-FK cv-PDG FLARE™ Assay Kit, 75 Samples
4065-100-FM cv-PDG FLARE™ Module, 100 Samples


UVDE FLARE™ Assay Kit and Module

UVDE FLARE™ Assay Kit and Module
 
Trevigen's UVDE FLARE (Fragment Length Analysis using Repair Enzymes) Assay provides the ability to probe for DNA damage in single cells using S. pombe UVDE in conjunction with Trevigen's CometAssay™ single cell gel electrophoresis kit. The UVDE FLARE Assay Kit supplies the enzyme, reagents, and FLARE Slides to perform the CometAssay'. The FLARE Module only provides the enzyme and buffers for use with the CometAssay.
 
Components: UVDE FLARE™ Assay Kit
  Catalog # Component Size
  4100-100-01
 		 S. pombe UVDE GST-.228 100 µl
  3951-040-01 25X FLARE Buffer 2 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-100-05 100X Cation Solution 100 µ
  3950-075-02 FLARE Slides 25 each
  4250-050-01 Lysis Solution 2 x 500 ml
  4250-050-02 Comet LMAgarose (LMA) 15 ml
  4250-050-05 **SYBR® Green I nucleic acid gel stain 5 µl
   
Components: UVDE FLARE™ Assay Kit
  Catalog # Component Size
  4100-100-01
 		 S. pombe UVDE GST-.228 100 µl
  3951-040-01 25X FLARE Buffer 2 40 ml
  3950-010-03 REC™ Dilution Buffer 10 ml
  3950-100-04 100X BSA Additive 100 µl
  3950-100-05 100X Cation Solution 100 µ
       
** SYBR® Green I nucleic acid gel stain is a registered Trademark of Molecular Probes inc, Eugene, OR. For license terms, please click here.

Products

4100-100-FK UVDE FLARE™ Assay Kit, 75 Samples
4100-100-FM UVDE FLARE™ Module, 100 Samples

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07