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Cultrex® Calcein-AM Cell Viability Assay
| The Calcein-AM Kit provides a
simple, rapid and accurate method to measure cell viability and/or
cytotoxicity. Calcein-AM is a non-fluorescent, hydrophobic compound that
easily permeates intact, live cells. The hydrolysis of Calcein-AM by
intracellular esterases produces calcein, a hydrophilic, strongly
fluorescent compound that is well-retained in the cell cytoplasm. Cells
grown in black-walled plates can be stained and quantified in less than
two hours. |
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Suitable for
proliferating and non-proliferating cells
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Ideal for both suspension and adherent cells
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Non-radioactive microplate
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Rapid (no solubilization step as in an MTT
assay)
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Ideal for high-throughput assays
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Better retention and brightness compared to
other fluorescent compounds (i.e.
fluorescein)
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Useful in a variety of studies, including:
cell adhesion, chemotaxis, multidrug
resistance, cell viability, apoptosis, and
cytotoxicity
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Adaptable to a wide variety of techniques,
including: microplate assays (described
here), immunocytochemistry, flow cytometry,
and in vivo cell tracing |
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Cell viability assays
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Cytotoxicity
analysis
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| Catalog # |
Component |
Size |
4892-010-01
|
Calcein-AM |
2 x 50 µg
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| 4892-010-02 |
10X Calcein-AM DW Buffer
|
200 ml |
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| Components are stored at -20°C and
4°C. |
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Products
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4892-010-K |
Calcein-AM Cell Viability Kit, 1000 Tests |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45