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Cultrex® 96 Well Collagen I Cell Invasion Assays
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Cultrex® Cell Invasion Assays were created in an effort to accelerate
the screening process for compounds that influence cell migration
through extracellular matrices. These assays offer a flexible,
standardized, high-throughput format for quantitating the degree to
which invasive cells penetrate a barrier consisting of basement membrane
components in vitro in response to chemoattractants and/or inhibiting
compounds. The Cultrex® Cell Invasion Assay has been adapted to multiple
formats so that cell invasion may be evaluated against different
extracellular matrices; the assay is available in the following formats:
Basement Membrane Extract (BME), Laminin I, Collagen I, Collagen IV. |
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| Cell
migration/Invasion Assays |
|
| Catalog # |
Component |
Size |
| |
3457-096-K
|
Collagen I Cell
Invasion Assay |
96 samples |
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3455-096-01 |
Cell Invasion
Chamber |
each |
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3457-096-02 |
10X Collagen I
Solution |
1 ml |
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3455-096-03 |
10X Coating
Solution |
1 ml |
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3455-096-04 |
25X Cell Wash
Buffer |
2 ml |
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3455-096-05 |
10X Cell
Dissociation Solution |
2 ml |
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4892-010-01 |
Calcein AM |
50 µg |
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| Components are stored at -20ºC,
4ºC, and at room temperature. |
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Liotta, L.A. 1984. Tumor invasion
and metastasis: role of the basement membrane. Am. J. Pathol.
117:339-348.
Terranova, V.P., E.S. Hujanen, D.M. Loeb, G.R. Martin, L.
Thornburg, and V. Glushko. 1986. Use of reconstituted basement
membrane to measure cell invasiveness and select for highly
invasive tumor cells. Proc. Natl. Acad. Sci. USA83:465-469.
Albini, A., Y. Iwamoto, H.K. Kleinman, G.R. Martin, G.R.
Aaronson, J.M. Kozlowski, and R.N. McEwan. 1987. A rapid in
vitroassay for quantitating the invasive potential of tumor
cells. Cancer Res. 47:3239-3245. |
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Products
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3457-096-K |
Cultrex®
96 Well Collagen I Cell Invasion Assay, 96 samples |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45