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Cultrex® 96 Well BME Cell Invasion Assays
| Cultrex® Cell Invasion Assays were
created in an effort to accelerate the screening process for compounds
that influence cell migration through extracellular matrices. These
assays offer a flexible, standardized, high-throughput format for
quantitating the degree to which invasive cells penetrate a barrier
consisting of basement membrane components in vitro in response to
chemoattractants and/or inhibiting compounds. The Cultrex® Cell Invasion
Assay has been adapted to multiple formats so that cell invasion may be
evaluated against different extracellular matrices; the assay is
available in the following formats: Basement Membrane Extract (BME),
Laminin I, Collagen I, Collagen IV. |
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|
| Cell
migration/Invasion Assays |
|
| Catalog # |
Component |
Size |
| |
3455-096-K
|
BME Cell Invasion
Assay |
96 samples |
| |
3455-096-01 |
Cell Invasion
Chamber |
each |
| |
3455-096-02 |
5X BME Solution
|
1 ml |
| |
3455-096-03 |
10X Coating
Solution |
1 ml |
| |
3455-096-04 |
25X Cell Wash
Buffer |
2 ml |
| |
3455-096-05 |
10X Cell
Dissociation Solution |
2 ml |
| |
4892-010-01 |
Calcein AM |
50 µg |
| |
*Basement
Membrane Extract (BME) |
| |
| Components are stored at -20ºC,
4ºC, and at room temperature. |
| |
Liotta, L.A. 1984. Tumor invasion
and metastasis: role of the basement membrane. Am. J. Pathol.
117:339-348.
Terranova, V.P., E.S. Hujanen, D.M. Loeb, G.R. Martin, L.
Thornburg, and V. Glushko. 1986. Use of reconstituted basement
membrane to measure cell invasiveness and select for highly
invasive tumor cells. Proc. Natl. Acad. Sci. USA83:465-469.
Albini, A., Y. Iwamoto, H.K. Kleinman, G.R. Martin, G.R.
Aaronson, J.M. Kozlowski, and R.N. McEwan. 1987. A rapid in
vitroassay for quantitating the invasive potential of tumor
cells. Cancer Res. 47:3239-3245. |
|
Products
|
3455-096-K |
Cultrex®
96 Well BME Cell Invasion Assay, 96 samples |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45