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Cultrex® 3D Culture Matrix™ Laminin I
| 3-D Culture is an innovative
approach to modeling the morphological effects of early oncogenesis on
glandular epithelial cells. When healthy, these cells exhibit a
structured, polarized morphology that is critical for cellular formation
and function. During carcinoma development, cell cycle controls
associated with cellular development, proliferation and death are lost,
and as a result, these ascinaric structures are disrupted. In effect,
the morphology of these structures can be used as a measure to study
factors in early carcinoma development. Trevigen has developed the 3D
Culture Matrix™ product line to
provide reagents specifically produced for and qualified in 3-D culture
studies. |
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| The 3-D Culture
Matrix™ Laminin I may be used as a gel on which to grow cells or
a media additive alone or in concert with other basement
membrane components to study cellular growth and differentiation
in three dimensions in vitro. |
| 3-D Culture Matrix™ Laminin I is provided in
DMEM medium containing 10 µg/ml gentamycin sulfate and no phenol
red at a concentration of ~ 3mg/ml. |
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| Purified from murine EHS sarcoma. |
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| Store at -20° C or -80° C in a
manual defrost freezer. Avoid repeated freeze-thaw cycles. |
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| Debnath J, Muthuswamy SK, Brugge
JS: Morphogenesis and oncogenesis of MCF-10A mammary epithelial
acini grown in three-dimensional basement membrane cultures.
Methods, 2003 pp. 256-268.
Webber MM, Bello D, Kleinman HK, and Hoffman MP. Acinar
differentiation by non-malignant immortalized human prostate
epithelial cells and its loss in malignant cells.
Carcinogenesis. 1997. Jun; 18(6): 1225-1231. |
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Products
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3446-005-01 |
3-D
Culture Matrix™ Laminin I, 5 ml |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45