|
| |
CometAssay™ Single Cell Gel Electrophoresis
CometAssay™ Kit
CometAssay™ 96
CometAssay™ Silver Staining Kit
Higher Throughput CometAssay™
DASH™
(Diffusion Apoptosis Slide Halo) Assay Kit
| Trevigen’s
CometAssay™ provides reagents
and our exclusive CometSlide™
for the rapid analysis of DNA fragmentation associated with DNA
damage. The comet assay or single cell gel electrophoresis assay
is based on the alkaline lysis of labile DNA at sites of damage.
The unwound, relaxed DNA is able to migrate out of the cell
during electrophoresis and can be visualized using **SYBR®
Green I nucleic acid gel stain. Cells that have accumulated DNA
damage appear as fluorescent comets with tails of DNA
fragmentation or unwinding, whereas, normal undamaged DNA does
not migrate far from the origin. The CometAssay is provided with
our exclusive CometSlides which greatly simplify the assay. Each
slide provides two sample surfaces specially treated to promote
agarose adherence, and a hydrophobic barrier to allow treatment
with one of Trevigen’s DNA repair enzymes. Simply add your cells
to the low melting point Comet LMAgarose, and pipet onto the
slide. |
| |
| • |
Detect and
quantitate DNA damage |
| • |
Identify
apoptotic cells quickly and easily |
| • |
Follow DNA
repair |
| • |
Higher
Throughput CometAssay |
|
| |
| • |
CometSlides
allow direct application of Comet LMAgarose without base
layers or pretreatment. |
| • |
Hydrophobic
barrier allows easy application of cells directly to
slide, and treatment with any of several Trevigen DNA
repair enzymes. |
| • |
Easy to use
kit format. |
|
| |
|
Cat# |
Component |
Size |
| |
4250-050-01 |
Lysis Solution |
2
x 500 ml |
| |
4250-050-02
|
Comet LMAgarose |
15 ml |
| |
4250-050-03 |
CometSlide |
25
ea |
| |
4250-050-04 |
200 mM EDTA |
12.5 ml |
| |
4250-050-05 |
**SYBR®
Green I nucleic acid gel stain |
5
µl |
| |
|
|
|
| Components are stored
at -20°C or room temperature.
|
| |
|
|
|
|
Products
|
4250-050-K |
CometAssay™ Kit, 50 Samples |
|
4250-050-03 |
CometSlides™, 25 Slides |
|
4250-200-03 |
CometSlides™, 100 Slides |
| Trevigen’s
CometAssay™ 96 uses our
exclusive CometSlide™ 96 that
is specially treated to promote adherence of low melting point
agarose. This eliminates the time consuming and unreliable
traditional method of preparing base layers of agarose. The use
of CometSlide 96 shortens assay time, and allows the rapid and
reliable analysis of large numbers of samples. Trevigen also
provides **SYBR® Green I for
DNA visualization providing improved sensitivity compared to
ethidium bromide. The CometAssay may be coupled with Trevigen’s
FLARE™ (Fragment Length
Analysis using Repair Enzymes) Assay that provides the added
ability to probe for specific types of DNA damage using DNA
repair glycosylases. Contact Trevigen for more details about
analysis of DNA damage and repair. |
| |
| • |
Allows the
rapid and reliable analysis of large numbers of samples. |
| • |
CometSlides™
allow direct application of Comet LMAgarose without base
layers or pretreatment. |
| • |
Hydrophobic
barrier allows easy application of cells directly to
slide, and treatment with any of several Trevigen DNA
repair enzymes. |
| • |
Easy to use
kit format. |
|
| |
| • |
For use with
Trevigen’s CometAssay™
and FLARE assay reagents and protocol |
| • |
Detect and
quantitate DNA damage |
| • |
Identify
apoptotic cells quickly and easily |
| • |
Follow DNA
repair |
|
| |
|
Cat# |
Component |
Size |
| |
4250-010-01 |
Lysis Solution |
100 ml |
| |
4250-050-02
|
Comet LMAgarose (LMA) |
15 ml |
| |
4253-096-03 |
Trevigen CometSlide™
96 |
2
each |
| |
4250-050-04 |
200 mM EDTA, pH 10 |
12.5 ml |
| |
4250-050-05 |
**SYBR®
Green I nucleic acid gel stain |
5
µl |
| |
|
|
|
| Components are stored
at -20°C and room temperature. |
| |
|
|
|
|
Products
|
4253-096-K |
CometAssay™ 96, 192 Samples |
|
4253-096-03 |
96-Well CometSlide™, 1 Slide |
|
4253-960-03 |
96-Well
CometSlide™, 10 Slides |
|
4253-02K-03 |
96-Well
CometSlide™, 20 Slides |
|
4253-10K-03 |
96-Well
CometSlide™, 100 Slides |
Trevigen’s
CometAssay™ Silver Staining
Kit is designed for the convenient silver staining of CometAssay™
or single cell gel electrophoresis results. Recording of data
generated by the CometAssay™
and staining with fluorescent dyes requires epifluorescence
microscopy and rapid analysis because of the generally short
lifespan of the dye. Using the Silver Staining kit, permanent
records are created and visualization using standard light
microscopy is possible. The Silver Staining Kit was designed
specifically for use with the CometSlides™
to minimize unwanted background and the amount of hazardous
waste generated by silver nitrate.
|
| |
|
| • |
Convenient
format. |
| • |
Optimized for
CometSlide staining. |
| • |
Reduced
hazardous waste. |
|
| |
|
| • |
CometAssay™
 |
| • |
DNA Damage
detection |
|
| CometAssay™
Silver Staining Kit |
|
Catalog # |
Component |
Size |
| |
4254-200-01
|
20X Staining Reagent #1 |
1.2 ml |
| |
4254-200-02 |
20X Staining Reagent #2 |
1.2 ml |
| |
4254-200-03 |
20X Staining Reagent #3 |
1.2 ml |
| |
4254-200-04* |
2X Staining Reagent #4 |
1.2 g |
| |
4254-200-05 |
10X Fixation Additive |
2.2 ml |
| |
| CometAssay™
Silver |
|
Catalog # |
Component |
Size |
| |
4250-050-01
|
Lysis Solution |
2x500 ml |
| |
4250-050-02 |
Comet LMAgarose |
15 ml |
| |
4250-050-03 |
CometSlide |
25 ea |
| |
4250-050-05 |
200 mM EDTA |
12.5 ml |
| |
4254-200-K** |
Components |
|
| Store (cat#
4254-200-K) at room temperature.
|
|
Products
|
4254-200-K |
CometAssay™ Silver Staining Kit, 200 Samples |
|
4251-050-K |
CometAssay™ Silver (CommetAssay Kit w/silver staining reagents), 50
Samples |
| Trevigen’s
CometSlide™ HT provides a
convenient higher throughput, simple and reliable sample
preparation, processing, analysis and storage format for the
comet assay. Trevigen’s CometAssay™
uses our exclusive CometSlide™
HT that is specially treated to promote adherence of low melting
point agarose. This eliminates the time consuming and unreliable
traditional method of preparing base layers of agarose. The use
of CometSlide™ HT shortens
assay time, and allows the rapid and reliable analysis of large
numbers of samples. Trevigen’s
CometAssay™
provides reagents and our exclusive CometSlide™
for the rapid analysis of DNA fragmentation associated with DNA
damage. The comet assay or single cell gel electrophoresis assay
is based on the alkaline lysis of labile DNA at sites of damage.
The unwound, relaxed DNA is able to migrate out of the cell
during electrophoresis and can be visualized by **SYBR®
Green I nucleic acid gel stain. Cells that have accumulated DNA
damage appear as fluorescent comets with tails of DNA
fragmentation or unwinding, whereas, normal undamaged DNA does
not migrate far from the origin. The CometAssay™
is provided with our exclusive CometSlides™
HT which greatly simplify the assay. Each slide provides 20
sample surfaces specially treated to promote agarose adherence,
and a hydrophobic barrier to allow treatment with one of
Trevigen’s DNA repair enzymes. Simply add your cells to the low
melting point Comet LMAgarose, and pipet onto the slide. |
| |
| • |
Allows the
rapid and reliable analysis of large numbers of samples. |
| • |
CometSlides™
allow direct application of Comet LMAgarose without base
layers or pretreatment. |
| • |
Hydrophobic
barrier allows easy application of cells directly to
slide, and treatment with any of several Trevigen DNA
repair enzymes. |
| • |
Easy to use
kit format. |
|
| |
| • |
For use with
Trevigen’s CometAssay™
and FLARE assay reagents and protocol |
| • |
Detect and
quantitate DNA damage |
| • |
Identify
apoptotic cells quickly and easily |
| • |
Follow DNA
repair |
|
| |
|
| |
| Store at room
temperature with desiccant.
|
| |
|
|
|
|
Products
|
4252-040-01 |
CometAssay™ HT, 2 Slides |
|
4252-040-K |
CometAssay™ HT Kit, 40 Samples |
|
4252-200-01 |
CometAsssay™ HT, 10 Slides |
|
4252-500-01 |
CometAsssay™ HT, 25 Slides |
|
4252-02K-01 |
CometAssay™ HT, 100 Slides |
| Trevigen's new
comet assay kit, DASH™
(Diffusion Apoptosis Slide Halo), allows discrimination of
healthy and damaged cells in just a few hours. Simply embed
cells in low melting agarose on a pre-treated slide, lyse under
alkaline conditions, and precipitate the DNA of the remaining
nuclear region (i.e. nucleoid) in the agarose. The precipitated
DNA is visualized using **SYBR®
Green I nucleic acid stain. When excited (425 - 500 nm) the
DNA-bound **SYBR® Green I
emits green light. In healthy cells, the fluorescence is
confined to the nucleoid: undamaged DNA is supercoiled and thus
does not diffuse very far, if at all, from the nucleoid. In
cells that have accrued damage to the DNA, the alkali lysis
treatment unwinds the DNA, releasing fragments that diffuse away
from the center of the nucleoid, creating a "halo" surrounding a
small compact origin. This kit includes ready to use, pre-coated
slides and optimized reagents for your convenience. |
| |
| • |
Detect and
quantitate DNA damage |
| • |
Identify
apoptotic cells quickly and easily |
| • |
Follow DNA
repair |
|
| |
| • |
Hydrophobic
barrier allows easy application of cells directly to
slide, and treatment with any of several Trevigen DNA
repair enzymes. |
| • |
Easy to use
kit format. |
| • |
Work with a
small number of cells. |
|
| |
|
Cat# |
Component |
Size |
| |
4250-050-03 |
CometSlides™ |
25
slides |
| |
4255-050-02
|
LMAgarose-DQ |
15 ml |
| |
4255-050-01 |
Lysis solution-DQ |
2
x 500 ml |
| |
4255-050-04 |
100X NP Buffer-DQ |
20 ml |
| |
4250-050-05 |
**SYBR®
Green I |
5
µl |
| |
|
|
|
| Components are stored
at -20°C or room temperature. |
| |
| Singh NP. (2000) A
simple method for accurate estimation of apoptotic cells.
Exp Cell Res. 256(1): 328-37
Singh NP, Muller CH, Berger RE. (2003) Effects of age on DNA
double-strand breaks and apoptosis in numan sperm. Fertil,
Steril. 80(6): 1420-30. |
| |
|
Products
|
4255-050-K |
DASH™
Kit, 50 Samples |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45