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Cell Proliferation
TACS™ Hoechst Cell Proliferation Assays
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| TACS™
MTT Cell Proliferation Assay |
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| The TACS MTT Cell Proliferation
Assay (MTT-CPA) is a sensitive kit for the measurement of cell
proliferation based upon the reduction of the tetrazolium salt
3,[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT).
Changes in cell proliferative activity caused by trophic factors, growth
inhibitors, or inducers and inhibitors of apoptosis, may be quantified
using the MTT-CPA. MTT is reduced to an insoluble formazan dye by
mitochondrial enzymes associated with metabolic activity. The reduction
of MTT is primarily due to glycolytic activity within the cell and is
dependent upon the presence of NADH and NADPH.
Common methods for determining cell viability depend upon membrane integrity (e.g. trypan blue exclusion), or incorporation of nucleotides during cell proliferation (e.g. BrdU or 3H-thymidine). These methods are limited by the impracticality of processing large numbers of samples, or by the requirement for handling hazardous materials. The MTT Assay, in contrast, provides a rapid and versatile method for assessing cell viability. The assay is used to measure changes in cell proliferation. In actively proliferating cells, an increase in MTT conversion is spectrophotometrically quantified. Comparison of this value to an untreated control provides a relative increase in cellular proliferative activity. Conversely, in cells that are undergoing apoptosis, MTT reduction decreases, reflecting the loss of cell viability. |
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Products
| 4890-25-K | MTT Cell Proliferation Assay, 2500 Tests |
| 4890-50-K | MTT Cell Proliferation Assay, 5000 Tests |
| TACS™
XTT Cell Proliferation Assay |
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| The use of tetrazolium salts,
including XTT (2,3-Bis(2-methoxy-4-nitro-5-
sulfophenyl)-2H-tetrazolium-5-carboxanilide), to assay cell
proliferation, cell viability, and/or cytotoxicity is a widespread,
established practice. The procedures avoid radioactivity, allow for
rapid determination in microplates, and give reproducible and sensitive
results.
Cleavage of the tetrazolium salt to formazan occurs via the succinatetetrazolium reductase system in the mitochondria of metabolically active cells. The reaction is attributed mainly to mitochondrial enzymes and electron carriers, but a number of other non-mitochondrial enzymes have been implicated. XTT, a yellow tetrazolium salt, is cleaved to a soluble orange formazan dye, which can be measured by absorbance at 490 (or 450) nm in a microplate reader. Efficient reduction of XTT requires an electron coupling reagent. This kit includes both XTT and the electron coupling reagent for a convenient and simple assay. |
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Products
| 4891-025-K | TACS™ XTT Cell Proliferation Assay, 2500 Tests |
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