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Apoptosis-Related Antibodies
Caspase 2 Polyclonal
Antibody
Cleaved Caspase 3
Polyclonal Antibody
Caspase 7 Monoclonal
Antibody
Caspase 9 Monoclonal
Antibody
Caspase 10/a
(Mch 4) Monoclonal Antibody
Caspase 10/b
(Flice 2) Polyclonal Antibody
CD95 (Fas)/TNFRSF6
Antibodies
APAF-1 Monoclonal Antibody
Anti-G3PDH Polyclonal
Antibody
IAP Polyclonal Antibodies
Anti-TRAIL Polyclonal
Antibody
Anti-XIAP Polyclonal
Antibody
| This antibody is a
rabbit polyclonal antibody generated by immunization with
the KLH-coupledsynthetic peptide corresponding to amino
acids 414 - 428 of mouse caspase 2. |
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| This antibody
recognizes human and mouse procaspase 2 and the small
caspase 2 subunit that is generated during proteolytic
cleavage. |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
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Products
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2304-PC-040 |
Anti-human/ mouse Caspase 2 Polyclonal Antibody, 40 µg |
Following
induction of apoptosis, proteolytic cleavage of procaspase-3
occurs to generate an active 18 kDa caspase-3 fragment which
targets key modulators of the apoptotic pathway, including
poly-ADP-ribose polymerase and other caspases, for cleavage.
Trevigen’s antibody recognizes the active 18 kDa fragment
but does not recognize the 32 kDa procaspase-3, and is
therefore useful as a specific marker for apoptosis.
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| This antibody is a
rabbit polyclonal generated by immunization with a 13-amino
acid peptide sequence from carboxyl terminus of the human
and mouse caspase-3 p18 subunit. |
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| The antibody
detects human and mouse cleaved caspase-3. |
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| This antibody is provided as
affinity purified IgG fraction in phosphate buffered saline
with 0.01% sodium azide. |
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• Immunocytochemistry
• Western blotting
• Immunoprecipitation |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings.s. |
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Products
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2305-PC-020 |
Anti-Cleaved Caspase 3 Rabbit Polyclonal Antibody,20 µl |
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2305-PC-100 |
Anti-Cleaved Caspase 3 Rabbit Polyclonal Antibody,100 µl |
| Anti-caspase 7 was
raised against a synthetic peptide corresponding to amino
acids 1 to 32 of the human caspase 7 sequence. |
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| The antibody
recognizes human caspase 7. |
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| The antibody is affinity
purified and provided in phosphate buffered saline
containing 0.01% sodium azide. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
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2312-MC-050 |
Anti-Caspase 7 Monoclonal Antibody (clone MCH3-5) 50 µgl |
Caspase 9 is
an effector caspase involved in the activation of caspases
including caspase 3. The procaspase 9 binds ATP and
cytochrome c, and subsequently proteolytically cleaves
itself to form the active caspase 9. Caspase 9 is thought to
be involved in mitochondrial regulation of apoptosis.
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| Anti-caspase 9 was
raised against recombinant human caspase-9 protein. |
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| The antibody
recognizes human caspase 9. |
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| The antibody is affinity
purified and provided in phosphate buffered saline
containing less than 0.1% sodium azide. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
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2308-MC-050 |
Anti-Caspase 9 Monoclonal Antibody (clone LAP6-96) 50 µg |
| The antibody was
raised against purified Escherichia coli-derived,
recombinant human Caspase 10/a (Mch 4). |
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| This antibody
recognizes human and mouse caspase 10. Sensitivity for
detection of the subunits are not known. |
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| IgG2b |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
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2309-MC-050 |
Anti-Human Caspase 10/a Monoclonal Antibody (clone 63131.111), 50 µg |
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The antibody is a rabbit polyclonal antibody raised against
the KLH-coupled synthetic peptide KRTVWGAKQISATSLPTAC
corresponding to amino acids 487 - 504 of human caspase 10/b
(FLICE 2). Cysteine was added to the carboxyl-terminal for
coupling to KLH and for coupling to an affinity matrix. |
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| This antibody
recognizes human and mouse caspase 10 precursor. Sensitivity
for detection of the small subunit is not known. |
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| The antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
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2310-PC-050 |
Anti-Human Caspase 10/b Polyclonal Antibody 50 µg |
| Apoptosis
can be induced by the engagement of the death receptor CD95
(APO-1/Fas). Signaling through Fas requires Fas aggregation
and the formation of an intracellular protein complex called
the death-inducing signaling complex. This complex consists
of the cytoplasmic domain of Fas, the adaptor molecule
FADD/MORT-1 and procaspase-8 (Mch5/Flice/Mach). |
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| Anti-human
CD95 (Fas)/TNFRSF6 Polyclonal Antibody |
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| This antibody was
raised in goat against recombinant human Fas (rhFas). |
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| The antibody
recognizes human Fas. |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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• Western blotting
• ELISA |
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| Anti-human
CD95 (Fas)/TNFRSF6 Monoclonal Antibody (Clone DX2) |
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| This antibody was raised
against CD95 transfected L cells. |
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| The antibody recognizes human
Fas. |
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| IgG1 |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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• Western blotting
• Immunohistochemistry
• ELISA
• Induction of apoptosis |
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| Anti-human
CD95 (Fas)/TNFRSF6 Monoclonal Antibody (Clone 50830.11) |
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This antibody was raised
against recombinant human Fas
(rhFas). |
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| The antibody recognizes human
Fas. |
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| IgG1 |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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• Western blotting
• ELISA |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
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Products
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2342-PC-050 |
Anti-human CD95 (Fas)/TNFRSF6 Polyclonal Antibody, 50 µg |
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2343-MC-250 |
Anti-human CD95 (Fas)/TNFRSF6 Monoclonal Antibody (clone DX2), 250
µg |
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2344-MC-250 |
Anti-human CD95 (Fas)/TNFRSF6 Monoclonal Antibody (Clone 50830.11),
250 µg |
APAF-1
(apoptotic protease activating factor-1) is a mammalian
homolog of CED-4 that regulates cell death by participating
in a ternary complex with cytochrome c, and procas-pase-9.
APAF-1 is a member of the CARD protein family, which
contains a caspase-recruitment domain linked to a
nucleotide-binding domain (NBD), regulating apoptosis and/or
NF-ê B activation. During apoptosis, release of cytochrome c
initiates dATP-dependent oligomerization of APAF-1 and
formation of the apoptosome caspase processing complex,
triggering the postmitochondrial-mediated effector caspase
cascade.
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| The antibody was
raised against amino acids 10-214 of recombinant APAF-1. |
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| Clone 94408.11
recognizes human APAF-1. |
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| IgG2b |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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| Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawings. |
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Products
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2340-MC-050 |
Anti-APAF-1 Monoclonal Antibody (clone 94408.11), 50 µg |
The
abundance of glyceraldehyde-3-phosphate dehydrogenase
(G3PDH) in eukaryotic cells is relatively unaffected by
external factors. In Western blot analysis the level of
G3PDH can be used as a reference value for comparison
between different cell lysates or gel loadings.
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| Anti-G3PDH is a
rabbit polyclonal antibody raised against a synthetic
peptide corresponding to a portion of the human
glyceraldehyde-3-phosphate dehydrogenase gene. |
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| The antibody
recognizes human and mouse G3PDH. |
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| The antibody is provided as
serum without preservatives. |
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• Western blotting
• Immunoprecipitation |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
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Products
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2275-PC-1 |
Anti-G3PDH Polyclonal Antibody, 100 µl |
| Members of
the IAP (Inhibitor of Apoptosis Protein) family were
initially identified in baculoviruses. IAPs are found in a
wide variety of organisms from vertebrates to insects
(Drosophila), and yeast to invertebrates (Caenorhabditis
elegans). XIAP, cIAP-1, and cIAP-2 are thought to inhibit
apoptosis through direct caspase inhibition. The
anti-apoptotic range of action of IAPs is broad and overlaps
with Bcl-2 family activity. All IAP proteins contain at
least one 70 amino acid baculovirus IAP repeats (BIR) which
is essential for their anti-cell death activity that is
thought to work by protein-protein interaction via zinc
binding domain. A common characteristic of the
caspase-binding IAP proteins (XIAP, cIAP-1 and cIAP-2) is
that they all exhibit three BIR domains in their sequence.
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| cIAP-1 is the mammalian IAP
homolog B (MIHB). This protein contains three tandem BIR
domains and is believed to bind specifically to caspase 3
and caspase 7 and not to the early apoptosis effector
caspases 8, 1, or 6. |
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| Anti-cIAP-1 is a
rabbit polyclonal antibody generated by immunization with a
KLH-coupled synthetic peptide corresponding to amino acids
527 to 546 of the human cIAP-1 sequence. |
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| The antibody
recognizes human and mouse cIAP-1, and will not recognize
cIAP-2 on Western blots. |
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| This antibody is affinity
purified and provided in phosphate buffered saline
containing 0.02% sodium azide. |
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| Western blotting |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
|
Products
|
2319-PC-050 |
Anti-cIAP-1 Polyclonal Antibody, 50 µg |
TRAIL (Tumor
Necrosis Factor-Related Apoptosis Inducing Ligand) is a cell
surface molecule involved in the initiation of apoptosis.
Known as a death receptor like Fas, TNFR-2, and DR-3, TRAIL
is activated upon ligand binding and engages the cells on
the cascade of signaling involving caspase activation
through an adapter molecule. TRAIL has been shown to induce
apoptosis in human melanoma cells and not in healthy cells
making it a prime candidate for cancer studies.
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| Anti-TRAIL is a
goat polyclonal antibody generated by immunization with
full-length recombinant human TRAIL. |
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| The antibody
recognizes and neutralizes human TRAIL. |
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| The antibody is affinity
purified and provided in sterile PBS (pH 7.4) containing
0.01% sodium azide. |
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• Western blotting
• Neutralization of activity
• ELISA |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
|
Products
|
2317-PC-050 |
anti-TRAIL Polyclonal Antibody, 50 µg |
| Members of
the IAP (Inhibitor of Apoptosis Protein) family were
initially identified in baculoviruses. IAPs are found in a
wide variety of organisms from vertebrates to insects
(Drosophila), yeast to invertebrates (Caenorhabditis
elegans). XIAP, cIAP-1, and cIAP-2 are thought to inhibit
apoptosis through direct caspase inhibition. The
antiapoptotic range of action of IAPs is broad and overlaps
with Bcl-2 family activity. All IAP proteins contain at
least one 70 amino acid baculovirus IAP repeats (BIR) which
is essential for their anti-cell death activity that is
thought to work by protein-protein interaction. A common
characteristic of the caspase-binding IAP protein (XIAP,
cIAP-1 and cIAP-2) is that they all exhibit three BIR
domains in their sequence.
Similarly to cIAP-1 and cIAP-2, XIAP (X Chromosome-Linked
Inhibitor of Apoptosis) is a mammalian IAP believed to be
involved in apoptosis inhibition through binding
specifically to caspase 3 and caspase 7. |
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| Anti-XIAP is a
goat polyclonal antibody corresponding to amino acids I-497
of the human XIAP sequence. |
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| The antibody
recognizes human, mouse, and rat XIAP proteins. |
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| The antibody is affinity
purified and provided in sterile PBS containing 0.02% sodium
azide. |
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| • Western blotting |
| |
| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
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Products
|
2323-PC-050 |
Anti-XIAP Polyclonal Antibody, 50 µg |
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