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Antibody to P53
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| The tumor suppressor
p53 plays a key role in DNA damage and repair, apoptosis and cell
growth. A variety of genotoxic stresses lead to stabilization and
activation of p53 that are mediated by multiple post-translational
modifications. The N-terminus of p53 may be heavily phosphorylated,
whereas the C-terminus may be phosphorylated, acetylated or sumoylated.
In vivo acetylation of the C-terminus of p53 by p300/CBP at
Lysine (K) 372, K373, K381, and K382, and in vitro by pCAF at
K317, has been linked to p53 activation, increased protein stability,
and optimal p53 transactivation. Several p53 deacetylases have been
identified including MDM2, a HDAC1-containing complex, and SIRT-2. |
Anti-Human p53 Polyclonal
Antibody
Anti-Human PPM1D/WIP1
Monoclonal Antibody
Anti-Human p53 AcK382
Polyclonal Antibody
Anti-Mouse p53 AcK379
Polyclonal Antibody
Anti-Mouse/Human p53 AcK317 Polyclonal Antibody
| The tumor suppressor p53 plays a key
role in DNA damage and repair, apoptosis and cell growth. A variety of
genotoxic stresses lead to stabilization and activation of p53 that are
mediated by multiple post-translational modifications. The N-terminus of
p53 may be heavily phosphorylated, whereas the C-terminus may be
phosphorylated, acetylated or sumoylated. In vivo acetylation
of the C-terminus of p53 by p300/CBP at Lysine (K) 372, K373, K381, and
K382, and in vitro by pCAF at K320, has been linked to p53
activation, increased protein stability, and optimal p53
transactivation. Several p53 deacetylases have been identified including
MDM2, a HDAC1-containing complex, and SIRT-2. |
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| The antibody was raised against a
peptide corresponding to amino acids 377 - 387 of the human
sequence. |
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| This antibody is ideal for use in
detection of total human p53. It can be used for
immunoprecipitation of total p53 from cell lysates for detection
of acetylated or any other modified p53 on immunoblotts.
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| The antibody is purified from
rabbit and is provided in phosphate buffered saline, 50%
glycerol. |
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| Western Blot of human p53.
Immunoprecipitation of p53 modified or unmodified. |
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| Store at -20°C in a manual defrost
freezer. |
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Products
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2373-PC-100 |
Anti-Human-p53 Polyclonal Antibody 100 µl |
| PPM1D/WIP1 is a serine/threonine
phosphatase that is a transcriptional target of p53 and is implicated in
cell cycle control, spermatogenesis, and lymphoid cell function.
PPM1D/WIP1 is known to directly inhibit phosphorylation of the p38
mitogen-activated (MAP) kinase protein. PPM1D/WIP1 mRNA is rapidly
induced in response to UV damage and the protein localizes to the
nucleus. PPM1D/WIP1 null mice have increased susceptibility to pathogens
and reduced male fertility and longevity. |
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| Synthetic peptide corresponding to
amino acids 40 to 59 of the human PPM1D/WIP1 protein sequence. |
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| Anti-PPM1D/WIP1 is specific for
human PPM1D/WIP1. |
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| This antibody is provided as
purified immunoglobulin from mouse ascites in 1X PBS without
preservative. |
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| Western blot, immunoprecipitation,
immunocytochemistry, and immunohistochemistry. |
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| Store at -20°C in a manual defrost
freezer. |
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Products
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2380-MC-100 |
Anti-Human PPM1D/WIP1 Monoclonal Antibody Clone WC10 100 µg |
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2380-250-01 |
Control
Cell Extract for Western Blot 250 µl |
| The tumor suppressor p53 plays a key
role in DNA damage and repair, apoptosis and cell growth. A variety of
genotoxic stresses lead to stabilization and activation of p53 that are
mediated by multiple post-translational modifications. The N-terminus of
p53 may be heavily phosphorylated, whereas the C-terminus may be
phosphorylated, acetylated or sumoylated. In vivo acetylation
of the C-terminus of p53 by p300/CBP at Lysine (K) 372, K373, K381, and
K382, and in vitro by pCAF at K320, has been linked to p53
activation, increased protein stability, and optimal p53
transactivation. Several p53 deacetylases have been identified including
MDM2, a HDAC1-containing complex, and SIRT-2. |
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|
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| The antibody was
raised against a peptide corresponding to amino acids 377 - 387
of the human sequence with an acetylated lysine at position 382.
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| This antibody is
ideal for use in detection of human p53 acetylated at Lysine 382
following immunoprecipitation of total p53 from cell lysates. In
ELISA, there is no detectable cross-reaction with unmodified
peptide. |
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| The antibody is purified from
rabbit and is provided in phosphate buffered saline, 50%
glycerol. |
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| Western Blot of immunoprecipitated
human p53. |
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| Store at -20°C in a manual defrost
freezer. |
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Products
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2371-PC-050 |
Anti-Human-p53 AcK382 Polyclonal Antibody 50 µl |
| The tumor suppressor p53 plays a key
role in DNA damage and repair, apoptosis and cell growth. A variety of
genotoxic stresses lead to stabilization and activation of p53 that are
mediated by multiple post-translational modifications. The N-terminus of
p53 may be heavily phosphorylated, whereas the C-terminus may be
phosphorylated, acetylated or sumoylated. In vivo acetylation
of the C-terminus of p53 by p300/CBP at Lysine (K) 372, K373, K381, and
K382, and in vitro by pCAF at K320, has been linked to p53
activation, increased protein stability, and optimal p53
transactivation. Several p53 deacetylases have been identified including
MDM2, a HDAC1-containing complex, and SIRT-2. |
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| The antibody was raised against a
peptide corresponding to amino acids 374-386 of the murine
sequence with an acetylated lysine at position 379. |
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| This antibody is ideal for use in
detection of mouse p53 acetylated at Lysine 379 following
immunoprecipitation of total p53 from cell lysates. In ELISA,
there is no detectable cross-reaction with unmodified peptide.
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| The antibody is purified from
rabbit and is provided in phosphate buffered saline, 50%
glycerol. |
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| Western Blot of immunoprecipitated
mouse p53. |
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| Store at -20°C in a manual defrost
freezer. |
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Products
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2370-PC-050 |
Anti-Mouse-p53 AcK379 Polyclonal Antibody 50 µl |
| The tumor suppressor p53 plays a key
role in DNA damage and repair, apoptosis and cell growth. A variety of
genotoxic stresses lead to stabilization and activation of p53 that are
mediated by multiple post-translational modifications. The N-terminus of
p53 may be heavily phosphorylated, whereas the C-terminus may be
phosphorylated, acetylated or sumoylated. In vivo acetylation
of the C-terminus of p53 by p300/CBP at Lysine (K) 372, K373, K381, and
K382, and in vitro by pCAF at K317, has been linked to p53
activation, increased protein stability, and optimal p53
transactivation. Several p53 deacetylases have been identified including
MDM2, a HDAC1-containing complex, and SIRT-2. |
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| The antibody was raised against a
peptide corresponding to amino acids 310-322 of the murine
sequence with an acetylated lysine at position 317. |
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| This antibody is ideal for use in
detection of p53 acetylated at Lysine 317 in mouse or Lysine 320
in human samples following immunoprecipitation of total p53 from
cell lysates. In ELISA, there is no detectable cross-reaction
with unmodified peptide. |
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| The antibody is purified from
rabbit and is provided in phosphate buffered saline, 50%
glycerol. |
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| Western Blot of immunoprecipitated
mouse or human p53. |
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| Store at -20°C in a manual defrost
freezer. |
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Products
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2372-PC-050 |
Anti-Mouse/ Human-p53 AcK317 Polyclonal Antibody 50 µl |
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+32 (0) 16
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+32 (0) 16
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