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Antibodies to Mitochondria-Related Proteins
Anti-Bax Monoclonal
Antibodies
Anti-Baxß Polyclonal Antibody
Anti-Bcl-2 Monoclonal
Antibodies
Anti-Bcl-XL Monoclonal
Antibodies
Anti-Bid Polyclonal Antibody
Anti-Bim Polyclonal Antibody
Anti-Cytochrome C
Monoclonal Antibodies
Anti-Mcl-1 Polyclonal Antibody
Anti-PBR Polyclonal Antibody
| Bax, a 21 kDa eukaryotic protein
plays an important role in the regulation of cell death in a number
of eukaryotic cells. The over-expression of Bax has been shown to
accelerate cell death. The ratio of Bax to other Bcl-2 family
members, and its subcellular distribution is thought to help
regulate the process of programmed cell death. |
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| Anti-Bax
YTH-5B7 |
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| The antibody was raised against
a synthetic peptide corresponding to amino acids 3 to 16 of
the mouse Bax sequence. |
| |
| The YTH-5B7 clone
recognizes both native and detergent-treated mouse Bax
protein. |
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| IgG1
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| Anti-Bax
YTH-6A7 |
| The antibody was raised against a synthetic
peptide corresponding to amino acids 12 to 24 of the human
Bax sequence. |
|
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| The YTH-6A7 clone is conformation dependent,
and will recognize only detergent-treated human, mouse, or
rat Bax protein and not the native form |
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| IgG1 |
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| Anti-Bax
YTH-2D2 |
| The antibody was raised against a synthetic
peptide corresponding to amino acids 3 to 16 of the human
Bax sequence. |
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| The YTH-2D2 clone will recognize both native
and detergent-treated human Bax protein. |
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| IgG1 |
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| The Bax antibodies are purified from mouse
ascites and are provided as 100 µg aliquots in PBS buffer
containing 0.01% sodium azide. |
|
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• Western blotting
• Immunoprecipitation
• Immunohistochemistry*
|
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| Store at 4°C. |
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Products
|
2280-MC-025 |
Anti-Mouse Bax Monoclonal Antibody clone YTH-5B7, 25 µg |
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2280-MC-100 |
Anti-Mouse Bax Monoclonal Antibody clone YTH-5B7, 100 µg |
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2281-MC-025 |
Anti-Bax Monoclonal Antibody clone YTH-6A7, 25 µg |
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2281-MC-100 |
Anti-Bax Monoclonal Antibody clone YTH-6A7, 100 µg |
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2282-MC-025 |
Anti-Human Bax Monoclonal Antibody clone YTH-2D2, 25µg |
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2282-MC-100 |
Anti-Human Bax Monoclonal Antibody clone YTH-2D2, 100µg |
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2325-MC-025 |
Anti-Mouse Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
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2330-MC-025 |
Anti-Human Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
| Bax is a 24 kDa protein that
shares the BH1, BH2, BH3 domain of Bax, but has a unique carboxyl
terminus that lacks the hydrophobic transmembrane domain. The
distribution of Bax mRNA differs from that of Bax RNA, and is
particularly abundant in the brain. |
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| The anti-Bax is a sheep
polyclonal antibody raised against a synthetic peptide
corresponding to amino acids 172 to 199 of the Bax protein. |
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| The antibody
recognizes human Baxß. |
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| The antibody is
affinity purified and provided in phosphate buffered saline
containing less than 0.1% sodium azide. |
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| Immunoprecipitation |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing |
|
Products
|
2283-PC-250 |
Anti-Bax Polyclonal Antibody, 250 µg |
| The Bcl-2 family of proteins
plays a crucial role in the regulation of cell death in many
eukaryotic systems. The over-expression of Bcl-2 has been shown to
promote cell survival and the ratio of Bcl-2 to other Bcl-2 family
members is believed to modulate the apoptotic process. |
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| Anti-Bcl-2
YTH-10C4 |
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| The antibody was raised against
a synthetic peptide corresponding to amino acids 61 to 76 of
the mouse Bcl-2 sequence. |
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| The YTH-10C4 clone
recognizes mouse and rat Bcl-2 protein. |
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| IgG1 |
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| Anti-Bcl-2
YTH-8C8 |
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| The antibody was raised against a synthetic
peptide corresponding to amino acids 41 to 54 of the human
Bcl-2 sequence. |
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| The YTH-8C8 clone recognizes
human Bcl-2 protein. |
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| IgG1 |
|
| The Bcl-2 antibodies are purified from mouse
ascites and are provided as 100 µg aliquots in PBS buffer
containing 0.01% sodium azide. |
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• Western blotting
• Immunoprecipitation |
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| Stable for at least 1 year when
stored at 4°C. |
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Products
|
2290-MC-025 |
Anti-Mouse Bcl-2 Monoclonal Antibody clone YTH-10C4, 25 µg |
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2290-MC-100 |
Anti-Mouse Bcl-2 Monoclonal Antibody clone YTH-10C4, 100 µg |
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2291-MC-025 |
Anti-Human Bcl-2 Monoclonal Antibody clone YTH-8C8, 25 µg |
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2291-MC-100 |
Anti-Human Bcl-2 Monoclonal Antibody clone YTH-8C8, 100 µg |
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2325-MC-025 |
Anti-Mouse Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
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2330-MC-025 |
Anti-Human Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
| The Bcl-2 family of proteins
plays a crucial role in the regulation of cell death in many
eukaryotic systems. Bcl-XL is a 28 kDa protein associated with cell
survival. Bcl-X is reported in two forms, Bcl-XL and Bcl-XS. The
short form is reported to inhibit the survival promoting activity of
the long form. Bcl-XS is a splice variant at the RNA level, but its
functional role is not clear. |
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| The antibody was raised against
a synthetic peptide corresponding to amino acids 3 to 14 of
the human Bcl-XL sequence. |
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| The YTH-2H12 clone
recognizes human, mouse and rat Bcl-XL protein. |
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| IgG2a |
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| The Bcl-XL antibody is purified from mouse
ascites and is provided as a 100 µg aliquot in PBS buffer
containing 0.01% sodium azide. |
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• Western blotting
• Immunohistochemistry
• Immunoprecipitation |
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| Store at 4°C. |
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Products
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2300-MC-025 |
Anti-Bcl-XL Monoclonal Antibody (clone YTH-2H12), 25 µg |
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2300-MC-100 |
Anti-Bcl-XL Monoclonal Antibody (clone YTH-2H12) 100 µg |
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2325-MC-025 |
Anti-Mouse Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
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2330-MC-025 |
Anti-Human Bcl-2 Family (Bax, Bcl-2, Bcl-XL) Panel, 25 µg each |
| Bid is a 24 kDa protein involved
in mitochondrial apoptotic events where the cleaved form inserts
into the mitochondria and triggers several downstream events. |
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| The anti-Bid
is a rabbit polyclonal antibody generated by immunization
with a KLH-coupled synthetic peptide corresponding to amino
acids 34 to 52 of the human Bid sequence plus a
carboxyl-terminal cysteine residue for coupling and
immunopurification. |
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| The antibody
recognizes human Bid protein. |
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| The antibody is affinity
purified and provided in sterile PBS buffer (pH 7.4)
containing 0.01% sodium azide. |
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| • Western blotting |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawing. |
|
Products
|
2315-PC-050 |
Anti-Bid Polyclonal Antibody, 50 µg |
| Bim (Bcl-2 interacting mediator
of cell death) is a proapoptotic protein sharing only the short BH3
domain (Bcl-2 Homology regions) with other members of the Bcl-2
family. Bim has three known isoforms, thought to be generated by
alternative splicing. Bim is essential for certain apoptotic
responses and to prevent overproduction of hematopoietic cells
suggesting that it may be a tumor suppressor. |
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The anti-Bim is a rabbit polyclonal antibody generated by
immunization with the KLH-coupled synthetic peptide
corresponding to amino acids 22 - 39 of human and mouse Bim
plus a carboxyl-terminal cysteine for coupling. |
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| This antibody
recognizes human and mouse Bim. |
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| This antibody is affinity
purified and provided in phosphate buffered saline without
preservative. |
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| • Western blotting |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
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Products
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2341-PC-050 |
Anti-Bim Polyclonal Antibody, 50 µg |
| Cytochrome c is a
well-characterized mitochondrial protein involved in cellular energy
metabolism. When released into the cytosol, cytochrome c can
activate the caspases responsible for apoptosis through interaction
with protease activating factors.
The precursor of cytochrome c, apocytochrome c, is synthesized in
the cytoplasm. Upon translocation into the mitochondria, cytochrome
c refolds and acquires a heme moiety required for functionality in
the mitochondrial respiration chain. The heme-bound form of
cytochrome c is called holocytochrome c. |
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|
Anti-Cytochrome C 4CYTC-21 |
| The antibody was
raised against cytochrome c purified from human heart. |
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| The antibody is
specific for denatured cytochrome c and apocytochrome c from
human, rat, murine, bovine, equine and porcine sources. |
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| IgG1 |
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| • Western blotting |
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|
Anti-Holocytochrome C 2CYTC-199 |
| The antibody was raised against
cytochrome c purified from human heart. |
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| The antibody is specific for
the heme-bound cytochrome c and will recognize native or
denatured human, rat, and murine holocytochrome c. It
recognizes only holocytochrome c and no other heme protein
(i.e. hemoglobin). This antibody does not cross react with
bovine, porcine or equine cytochrome c. |
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| IgG1 |
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| Both antibodies are mouse
monoclonal antibodies purified on protein G and are provided
frozen at 1 mg/ml in PBS. |
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| Recombinant apocytochrome c
(Cat# 6370-050-01) available separately. |
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| Freeze in working aliquots at
-20°C in a manual defrost freezer to avoid repeated
freeze-thawing. |
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Products
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6370-MC-100 |
Anti-Cytochrome C clone 4CYTC-21, 100 µg |
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6380-MC-100 |
Anti-Holocytochrome C clone 2CYTC-199, 100 µg |
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6370-050-01 |
Recombinant Apocytochrome C, 50 µl |
| Mcl-1 is a Bcl-2 homolog
involved in early induction of cellular differentiation. Cloned from
the human myeloblastic leukemia cell line, Mcl-1 is a 40 kDa
mitochondrial protein that has been shown to inhibit apoptosis
through the formation of dimers with Bcl-2 family member Bax. Mcl-1
is expressed in a wide variety of cancerous tissues. |
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|
Anti-Mcl-1 is a rabbit polyclonal antibody generated by
immunization with a KLH-coupled synthetic peptide
corresponding to amino acids 175 to 194 of the human Mcl-1
sequence. The murine sequence differs by a single amino acid
substitution of S for A at position 192 of human Mcl-1. |
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| The antibody
recognizes human Mcl-1. The antibody also detects a 27-34
kDa band in extracts of mouse L929 and DA3 cells believed to
be the murine form of Mcl-1. |
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| The antibody is affinity
purified and provided in sterile PBS (pH 7.4) containing
0.01% sodium azide. |
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| Western blotting |
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| Recombinant Murine Mcl-1 (Cat#
2316-050-01) available separately. |
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| Freeze in working aliquots at
-20 C in a manual defrost freezer to avoid repeated
freeze-thawing. |
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Products
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2316-PC-075 |
Anti-Mcl-1 Polyclonal Antibody, 75 µg |
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2316-050-01 |
Recombinant Murine Mcl-1, 50 µl |
| The peripheral-type
benzodiazepine receptor (PBR) is a ubiquitous protein involved in
the regulation of cholesterol transport from the outer to inner
mitochondrial membrane. Thought to play a role in the formation of
the mitochondrial permeability transition (PT) pore involved in
apoptotic mechanisms, PBR is also present in abundance in tumors, in
aggressive breast cancer cell lines, and in metastatic human breast
tumor biopsy sections. |
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The anti-PBR antibody was developed in rabbits against an
internal sequence of the PBR protein consisting of amino
acids 71 to 88 of the human PBR sequence. The specificity of
the antibody was assessed in steroidogenic cells and in
mitochondrial extracts. |
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| The antibody
recognizes mouse, rat, and human PBR protein. |
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| Anti-PBR antibody is provided
as rabbit anti-seral. |
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• Immunohistochemistry
• Western blotting
• Starting recommended dilution for Western blot is 1:1,000;
for immunohistochemistry, 1:250. |
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| Recombinant PBR protein (Cat#
6360-025-01) available separately. |
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| Stable for at least 3 years
when stored at -20°C. For long term storage, divide into
aliquots and avoid repeated freeze-thawing |
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Products
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6360-PC-100 |
Anti-PBR Antibody, 100 µl |
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6360-025-01 |
PBR
Protein (Recommend), 25 µl |
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