Antibodies to DNA Repair Enzymes & Related Enzymes

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Anti-AP Endonuclease Antibodies

Anti-AP Endonuclease/Ref-1 (APE/Ref-1) Antibodies

Human AP endonuclease (APE) is a multifunctional enzyme involved both in DNA repair and in facilitating the redox state of a number of DNA binding proteins. The DNA repair function of APE serves to cleave DNA at apurinic/apyrimidinic sites forming nicks in DNA which are subsequently repaired by DNA polymerases and ligases. The second, and seemingly unrelated function is the regulation of several transcription factors through the redox stimulation of DNA binding activity. These transcription factors include FOS, JUN, NF-KB, and p53. The APE-related DNA repair activity has been demonstrated to be inactivated by phosphorylation suggesting that this might be the mechanism by which the enzyme switches between the two functions. Additionally, expression levels of APE have been demonstrated to be associated with apoptosis induction.

Anti-Human AP Endonuclease/Ref-1 (APE/Ref-1)
Polyclonal Antibody (Cat#: 4415-PC-100)

Immunogen:

Full-length affinity purified APE/Ref-1 protein.

Specificity:

This antibody recognizes human APE/Ref-1 protein, and to a lesser extent mouse and rat APE/Ref-1 protein.

Preparation:

The antibody is affinity purified and provided in PBS buffer preserved using 0.01% sodium azide.

Applications:

•	Western blotting
•	Immunohistochemistry
•	Neutralization
•	Double labeling with TUNEL Assay

Storage:

Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.

Anti-Human AP Endonuclease/Ref-1 (APE/Ref-1)
Monoclonal Antibody (Cat#: 4417-MC-100)

Immunogen:

Recombinant human APE/Ref-1 protein.

Specificity:

This antibody recognizes human, mouse, and rat APE-1/Ref-1.

Preparation:

The antibody is affinity purified and provided in PBS containing 0.01% sodium azide.

Applications:

•	Western blotting
•	Immunohistochemistry

Storage:

Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.

Products

4415-PC-100

Anti-APE/Ref-1 Polyclonal Antibody, 100 µg

4417-MC-100

Anti-APE/Ref-1 Monoclonal Antibody, 100 µg

Anti-Fen-1 Polyclonal Antibody

The Fen-1 antibody recognizes mammalian flap-endonuclease (Fen-1). Fen-1 is involved in DNA double-strand break repair, and base excision repair. This antibody has been shown to inhibit Fen-1 activity.

Immunogen:

Full-length recombinant human Fen-1 protein.

Specificity:

This antibody recognizes human and mouse Fen-1 protein.

Preparation:

The antibody is provided as rabbit anti-sera.

Applications:

• Western blotting
• Neutralization

Storage:

Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawing.

Products

4410-PC-100

Anti-Fen-1 Polyclonal Antibody, 100 µl

Anti-Formamidopyrimidine DNA Glycosylase (Fpg) Rabbit Polyclonal Antibody

Anti-Formamidopyrimidine DNA Glycosylase (Fpg)
Rabbit Polyclonal Antibody

Fpg releases damaged bases, preferentially from duplex DNA. It has an associated class I AP lyase activity, leaving both 3’ and 5’ phosphoryl groups. This results from a β, δ delimination reaction at the AP sites, producing a single nucleotide gap in the DNA. Trevigen’s antibody recognizes 30.2 kDa Fpg recombinant protein.

Immunogen:

This antibody was raised against full-length recombinant Fpg protein.

Specificity:

This antibody recognizes E.coli Fpg.

Preparation:

The antibody is provided as rabbit anti-sera in phosphate buffered saline without preservative.

Applications:

•	Western blotting

Storage:

Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.

Products

4420-PC-100

Anti-Fpg Polyclonal Antibody, 100 µl

Anti-Rad1 Antibody (clone 33)

Anti-Human Rad1 Monoclonal Antibody (clone 33)

Cell cycle checkpoints in yeast and humans are activated by DNA damage. Rad1 was identified in yeast as a component of the checkpoint complex using checkpoint mutants. The human homolog of Rad1 has been shown to form a stable radioresponsive checkpoint complex with the human Rad9 and Hus1 proteins. This 32 kDa complex is present in undamaged cells but undergoes extensive modification in response to DNA damage. Human Rad1 exhibits homology with the Ustilago maydis Rec1 exonuclease and may possess a 3’- 5’ exonuclease activity.

Immunogen:

Full length recombinant human Rad1.

Specificity:

This antibody recognizes mouse and human Rad1.

IsoType:

IgG_2aκ

Preparation:

This antibody is provided as purified immunoglobulin from mouse ascites in PBS containing 0.01% sodium azide.

Applications:

•	Western blotting

Storage:

Freeze in working aliquots at -20°C in a manual defrost freezer to avoid repeated freeze-thawings.

Products

4451-MC-200

Anti-Human Rad1 Monoclonal Antibody (clone 33),200 µg

Antibodies to DNA Repair Enzymes & Related Enzymes

Anti-AP Endonuclease Antibodies

Anti-Fen-1 Polyclonal Antibody

Anti-Formamidopyrimidine DNA Glycosylase (Fpg) Rabbit Polyclonal Antibody

Anti-Rad1 Antibody (clone 33)

Send mail to webmaster with questions or comments about this web site. Copyright © 2005 Gentaur BVBA Last modified: feb-07

Send mail to webmaster with questions or comments about this web site.
Copyright © 2005 Gentaur BVBA
Last modified: feb-07