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Antibodies to DNA Damage
Anti-8-oxo-dG Monoclonal Antibody (Clone 1F7)
Anti-8-oxo-dG Monoclonal Antibody (Clone 4A6)
Anti-8-oxo-dG Monoclonal Antibody (Clone 4E9)
Anti-8-oxo-dG Monoclonal antibody (FAB 166)
| The anti-8-oxo-dG antibody allows
detection and quantitation of 7,8-dihydro-8-oxo-guanine (8-oxo-dG,
7,8-dihydro-8-oxo-guanine, or 7-oxo-7,8-dihydroguanine) in DNA, cell,
and tissue samples. The 8-oxo-dG lesion is the most common form of
base change caused by oxidative stress. Attack by free radical oxygen
occurs at the N7-C8 bond of guanine resulting in the formation of the
oxidized base.
DNA polymerases preferentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore, without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesions result in mutational frequencies of
1-5% (mainly G:C to T:A transitions).
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| 7,
8-dihydro-8-oxo-guanine. |
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|
| The
antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. Cross
reaction with guanine and guanosine occurs at concentrations 800
to 20,000-fold higher than for 7,8-dihydro-8-oxo-guanine. The
antibody will cross react with structurally related
7,8-dihydro-8-oxo-guanine derivatives, e.g. 5-
hydroxymethyluridine. Limited cross reaction with denatured calf
thymus has been observed, and as a result, this should be
avoided as a blocking agent in immunocytochemistry. |
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| IgG1 |
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| The
antibody is purified from mouse ascites and is provided in
phosphate buffered saline (PBS) containing 0.01% sodium azide. |
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| • |
Immunocytochemistry
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| • |
ELISA |
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| Store at 4°C.
For long term storage, divide into working aliquots and freeze
at -20°C in a manual defrost freezer to avoid repeated
freeze-thawings. |
|
Products
|
4355-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody clone 1F7,100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzeihmer's
disease, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo-dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond by oxidative
radicals. DNA polymerases preferentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore, without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1- 5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| Purified IgG is
provided in phosphate buffered saline with 0.01% sodium azide as
preservative. |
| |
|
| 7,
8-dihydro-8-oxo-guanine containing dinucleotide coupled to BSA. |
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| The antibody has
similar binding affinity for 8- hydroxydeoxyguanosine and for
7,8-dihydro-8-oxo guanine. This antibody recognizes the 8-oxoG
as a single nucleotide. |
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|
| IgG |
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|
| • |
Immunostaining
|
| • |
ELISA |
|
| Store at 4°C for
short term. For long term storage, divide into working aliquots
and freeze at -20°C in a manual defrost freezer to avoid
repeated freeze-thawings. |
|
Products
|
4359-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody (Clone 4E9), 100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzheimer's
disease¹, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo0dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond of guanine by
oxidative radicals. DNA polymerases prefentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1-5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| 7,
8-dihydro-8-oxo-guanine containing dinucleotide coupled to BSA |
| |
|
|
This antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. This
antibody recognizes the 8-oxo-G in single or double standard DNA
settings. |
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|
| IgG1 |
|
| |
|
| The
antibody is purified from mouse ascites and is provided in
phosphate buffered saline (PBS) containing 0.01% sodium azide as
a preservative. |
| |
|
| • |
Immunocytochemistry
|
| • |
ELISA |
|
| Store at 4°C.
For long term storage, divide into working aliquots and freeze
at -20°C in a manual defrost freezer to avoid repeated
freeze-thawings. Do not freeze and thaw aliquots more than once.
|
|
Products
|
4357-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody clone 4A6, 100 µg |
Mutagenic reactive oxygen species
are implicated in cancer, degenerative disorders such as Alzheimer's
disease¹, and in apoptosis. Oxidative damage can cause formation of
7,8-dihydro-8-oxo-guanine, often termed 8-oxo0dG. The production of
7,8-dihydro-8-oxo-guanine is almost exclusively elicited by oxidative
stress with the main attack site at the N7-C8 bond of guanine by
oxidative radicals. DNA polymerases prefentially insert adenine opposite
7,8-dihydro-8-oxo-guanine, therefore without repair these oxidative
damage adducts can lead to G to T transitions. The
7,8-dihydro-8-oxo-guanine lesion causes mutational frequencies of 1-5%
(mainly G:C to T:A transitions) and is one of the most abundant
oxidative lesions.
|
| Store at 4°C for
short term. Aliquot and freeze at -20°C for long term storage.
Do not freeze and thaw aliquots more than once. |
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| lgG, Fab fragment |
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| Purified Fab fragment provided in
PBS with 0.01% sodium azide as preservative. |
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| The
antibody has similar binding affinity for
8-hydroxydeoxyguanosine and for 7,8-dihydro-8-oxo-guanine. Cross
reaction with guanine and guanosine shown in Table 1 |
|
Products
|
4358-MC-100 |
Anti-8-oxo-dG Monoclonal Antibody FAB 166, 100 µg |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45